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用印度楝种子的非萜类提取物处理的培养小鼠成纤维细胞质膜的介电特性。

Dielectric properties of the plasma membrane of cultured murine fibroblasts treated with a nonterpenoid extract of Azadirachta indica seeds.

作者信息

Bonincontro Adalberto, Di Ilio Vincenzo, Pedata Osvaldo, Risuleo Gianfranco

机构信息

CNISM-Dipartimento di Fisica, Università di Roma La Sapienza, piazzale A. Moro 5, I-00185 Rome, Italy.

出版信息

J Membr Biol. 2007 Feb;215(2-3):75-9. doi: 10.1007/s00232-007-9007-2. Epub 2007 Apr 17.

DOI:10.1007/s00232-007-9007-2
PMID:17437145
Abstract

Neem oil is a natural product obtained from the seeds of the tree Azadirachta indica. In this report, we investigate the alterations of the biophysical properties of the plasma membrane caused by treatment with the nonterpenoid fraction of neem oil that we defined as methanolic extract (MEX). The dose-response effect was evaluated and a MEX-dependent cytoxicity evidenced. The effect of MEX on the plasma membrane was studied by a well-established dielectric spectroscopy technique: electrorotation, which allows single-cell analysis. Our results show a structural/functional alteration of the plasma membrane with an evident increase of specific capacitance and conductance. The biological implications of this effect are discussed.

摘要

印楝油是从印楝树的种子中提取的一种天然产物。在本报告中，我们研究了用印楝油的非萜类组分（我们定义为甲醇提取物，即MEX）处理后质膜生物物理特性的变化。评估了剂量反应效应，并证明了MEX依赖性细胞毒性。通过一种成熟的介电谱技术——旋转电泳来研究MEX对质膜的影响，旋转电泳可进行单细胞分析。我们的结果显示质膜存在结构/功能改变，比电容和电导率明显增加。讨论了这种效应的生物学意义。

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Dielectric properties of the plasma membrane of cultured murine fibroblasts treated with a nonterpenoid extract of Azadirachta indica seeds.用印度楝种子的非萜类提取物处理的培养小鼠成纤维细胞质膜的介电特性。
J Membr Biol. 2007 Feb;215(2-3):75-9. doi: 10.1007/s00232-007-9007-2. Epub 2007 Apr 17.
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Differential cytotoxicity of MEX: a component of Neem oil whose action is exerted at the cell membrane level.印楝素提取物的差异细胞毒性：印楝油的一种成分，其作用于细胞膜水平。
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本文引用的文献

1
Cytotoxic and antiproliferative effects induced by a non terpenoid polar extract of A. indica seeds on 3T6 murine fibroblasts in culture.印度楝种子的一种非萜类极性提取物对培养的3T6小鼠成纤维细胞诱导的细胞毒性和抗增殖作用。
Mol Cell Biochem. 2006 Jul;287(1-2):69-77. doi: 10.1007/s11010-005-9062-x. Epub 2006 May 2.
2
A combined dielectrophoresis, traveling wave dielectrophoresis and electrorotation microchip for the manipulation and characterization of human malignant cells.一种用于人类恶性细胞操控与表征的介电电泳、行波介电电泳和旋转电微芯片组合装置。
J Microbiol Methods. 2004 Sep;58(3):387-401. doi: 10.1016/j.mimet.2004.05.002.
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白藜芦醇在培养细胞中表现出很强的细胞毒性活性，并且对多瘤病毒具有抗病毒作用：潜在的临床用途。
J Exp Clin Cancer Res. 2009 Jul 1;28(1):96. doi: 10.1186/1756-9966-28-96.
4
Alterations of the plasma membrane caused by murine polyomavirus proliferation: an electrorotation study.鼠多瘤病毒增殖引起的质膜变化：一项旋转电泳研究。
J Membr Biol. 2009 May;229(1):19-25. doi: 10.1007/s00232-009-9172-6. Epub 2009 May 9.
5
Differential cytotoxicity of MEX: a component of Neem oil whose action is exerted at the cell membrane level.印楝素提取物的差异细胞毒性：印楝油的一种成分，其作用于细胞膜水平。
Molecules. 2008 Dec 31;14(1):122-32. doi: 10.3390/molecules14010122.
Neem--an omnipotent plant: a retrospection.
印楝——一种万能植物：回顾
Chembiochem. 2004 Apr 2;5(4):408-21. doi: 10.1002/cbic.200300749.
4
Variability in Neem (Azadirachta indica) with respect to azadirachtin content.印楝（Azadirachta indica）中印楝素含量的变异性。
J Agric Food Chem. 2003 Feb 12;51(4):910-5. doi: 10.1021/jf025994m.
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The effects of phytochemical pesticides on the growth of cultured invertebrate and vertebrate cells.植物化学农药对培养的无脊椎动物和脊椎动物细胞生长的影响。
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Cytotoxicity of azadirachtin A in human glioblastoma cell lines.印楝素A对人胶质母细胞瘤细胞系的细胞毒性。
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Biochimie. 2000 Jan;82(1):35-9. doi: 10.1016/s0300-9084(00)00358-8.
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Activity and biological effects of neem products against arthropods of medical and veterinary importance.印楝产品对具有医学和兽医学重要性的节肢动物的活性及生物学效应。
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