Jin T, Hu L-S, Chang M, Wu J, Winblad B, Zhu J
Department of Neurology, the First Hospital, Jilin University, Changchun, China.
Eur J Neurol. 2007 May;14(5):563-8. doi: 10.1111/j.1468-1331.2007.01761.x.
Increased protein level in the cerebrospinal fluid (CSF) is a characteristic of patients with Guillain-Barré syndrome (GBS), an acute inflammatory autoimmune disorder in the peripheral nervous system (PNS). However, the molecular mechanisms underlying the disease remain poorly understood and so far no reliable disease-related markers are available. By comparing the CSF proteome of GBS patients with control subjects suffering from other neurological disorders, it may be possible to identify proteins that involve in the disease process and thus to study the pathogenesis of GBS. We used two-dimensional difference gel electrophoresis (2D DIGE) technique, in combination with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), to determine the abnormal CSF proteins in GBS patients. Our data showed that the levels of six proteins and their isoforms in CSF were significantly altered in GBS patients compared with controls. Haptoglobin, apolipoprotein A-IV and PRO2044 (unnamed protein) were considerably increased in the CSF of GBS patients, whereas transthyretin, apolipoprotein E and fibrinogen were considerably decreased. We concluded that these six proteins may be involved in the pathogenesis of GBS and call for further studying the role of these proteins in the pathogenesis of the disease.
脑脊液（CSF）中蛋白质水平升高是吉兰-巴雷综合征（GBS）患者的一个特征，GBS是一种外周神经系统（PNS）的急性炎症性自身免疫性疾病。然而，该疾病的分子机制仍知之甚少，迄今为止尚无可靠的疾病相关标志物。通过比较GBS患者与患有其他神经系统疾病的对照受试者的脑脊液蛋白质组，有可能识别参与疾病过程的蛋白质，从而研究GBS的发病机制。我们使用二维差异凝胶电泳（2D DIGE）技术，结合基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS），来确定GBS患者脑脊液中的异常蛋白质。我们的数据显示，与对照组相比，GBS患者脑脊液中六种蛋白质及其异构体的水平有显著变化。GBS患者脑脊液中触珠蛋白、载脂蛋白A-IV和PRO2044（未命名蛋白质）显著增加，而转甲状腺素蛋白、载脂蛋白E和纤维蛋白原则显著减少。我们得出结论，这六种蛋白质可能参与了GBS的发病机制，并呼吁进一步研究这些蛋白质在该疾病发病机制中的作用。
