Unité de Biotechnologie des Algues, Biological Engineering Department, National School of Engineers of Sfax, University of Sfax, Sfax 3038, Tunisia.
Laboratoire de Biotechnologie Végétale Appliquée à l'Amélioration des Cultures, Faculty of Sciences of Sfax, University of Sfax, Sfax 3000, Tunisia.
Mar Drugs. 2017 Sep 20;15(9):293. doi: 10.3390/md15090293.
The green microalgae genus is known for the production of high added value molecules. In this study, strain AL-1 was isolated from the Sebkha of Sidi El Hani (Sousse, Tunisia). This isolate was identified both morphologically and genetically via 18S rRNA gene sequence as a member of the genus . Strain AL-1 was found to be closely related to , and with more than 97% similarity. Response surface methodology was used to maximize carotenoid production by strain AL-1 by optimizing its growth conditions. The highest carotenoid content was obtained at salinity: 51, light intensity: 189.89 μmol photons·m·s, and nitrogen: 60 mg·L. Proteomic profiling, using two-dimensional gel electrophoresis, was performed from standard and optimized cultures. We detected 127 protein spots which were significantly differentially expressed between standard and optimized cultures. Among them 16 protein spots were identified with mass spectrometry and grouped into different functional categories using KEGG (Kyoto Encyclopedia of Genes and Genomes) such as photosynthetic Calvin cycle, regulation/defense, energy metabolism, glycolysis, and cellular processes. The current study could be of great interest in providing information on the effect of stressful conditions in microalgae carotenoid production.
绿藻属以生产高附加值分子而闻名。在这项研究中，从西迪·埃勒哈尼盐沼（突尼斯苏塞）中分离出一株 AL-1 菌株。该分离株通过 18S rRNA 基因序列在形态和遗传上被鉴定为属的一个成员。AL-1 株与 、 和 密切相关，相似度超过 97%。响应面法被用于通过优化其生长条件来最大化 AL-1 菌株的类胡萝卜素产量。在盐度：51、光照强度：189.89 μmol 光子·m·s 和氮：60 mg·L 的条件下，获得了最高的类胡萝卜素含量。使用二维凝胶电泳进行了标准和优化培养物的蛋白质组学分析。我们在标准和优化培养物之间检测到 127 个明显差异表达的蛋白质斑点。其中 16 个蛋白质斑点被质谱鉴定，并使用 KEGG（京都基因与基因组百科全书）分为不同的功能类别，如光合作用卡尔文循环、调节/防御、能量代谢、糖酵解和细胞过程。本研究可以为微藻类胡萝卜素生产中应激条件的影响提供信息，具有重要意义。
