Spitz痣中的细胞周期和凋亡调节因子：与黑色素瘤和普通痣的比较。

Cell cycle and apoptosis regulators in Spitz nevi: comparison with melanomas and common nevi.

作者信息

Stefanaki Christina, Stefanaki Kalliopi, Antoniou Christina, Argyrakos Theodoros, Patereli Amalia, Stratigos Alexander, Katsambas Andreas

机构信息

Dermatology Department, Andreas Sygros University Hospital, Greece.

出版信息

J Am Acad Dermatol. 2007 May;56(5):815-24. doi: 10.1016/j.jaad.2006.09.015.

DOI:10.1016/j.jaad.2006.09.015

PMID:17437889

Abstract

BACKGROUND

Deregulated cell cycle control is one of the hallmarks of tumor development. The expression of different cell cycle regulators has been used in various neoplasms as an adjunct to diagnosis.

OBJECTIVE

We sough to determine the expression of cell cycle and apoptosis regulators in Spitz nevi and to appraise its value as a diagnostic adjunct in the differential diagnosis from melanomas and common nevi.

METHODS

Ki-67, p-27, p-16, p-53, p-21, Rb, cyclin D1, cyclin A, cyclin B1, bcl-2, and bax expression was assessed by immunohistochemistry in 10 Spitz nevi and was compared with 16 melanomas and 20 common nevi immunohistochemical expression.

RESULTS

P-27 (60% +/- 20.13), p-16 (62.00% +/- 10.85), and bcl-2 (46.00% +/- 42.47) were highly expressed in Spitz nevi, whereas Ki-67 (2.80% +/- 2.55), Rb (3.75% +/- 4.55), p-53 (2.30% +/- 0.10), cyclin A (0.70% +/- 1.56), B1 (0.20% +/- 0.34), and bax (2.65% +/- 6.37) demonstrated a limited expression. Cyclin D1 (8.60% +/- 7.30) and p-21 (6.40% +/- 5.37) showed a moderate expression. The expression of bax (P = .001), Ki-67 (P < .0001), Rb (P < .0001), p-16 (P < .0001), cyclin A (P < .0001), and cyclin B1 (P < .0001) was significantly higher in melanomas in comparison with Spitz nevi, whereas p-27 expression was significantly higher in Spitz nevi (P < .0001). A trend for significant difference in favor of melanomas was also observed for p-53 (P = .002). On the other hand, no difference was detected for bcl-2 (P = .275), p-21 (P = .055), or cyclin D1 (P = .077). Spitz nevi demonstrated a trend for a higher expression for p-21 (P = .008) and cyclin D1 (P = .006), whereas they exhibited lower p-16 (P = .004) in comparison with common nevi.

LIMITATIONS

The number of Spitz nevi was relatively small.

CONCLUSION

Spitz nevi differ from melanomas in their immunohistochemical pattern of expression of cell cycle and apoptosis regulators and more closely resemble common benign nevi.

摘要

背景

细胞周期调控失调是肿瘤发展的标志之一。不同细胞周期调节因子的表达已被用于各种肿瘤的辅助诊断。

目的

我们旨在确定Spitz痣中细胞周期和凋亡调节因子的表达，并评估其在与黑色素瘤和普通痣鉴别诊断中的辅助诊断价值。

方法

通过免疫组织化学法评估10例Spitz痣中Ki-67、p-27、p-16、p-53、p-21、Rb、细胞周期蛋白D1、细胞周期蛋白A、细胞周期蛋白B1、bcl-2和bax的表达，并与16例黑色素瘤和20例普通痣的免疫组织化学表达进行比较。

结果

Spitz痣中p-27（60%±20.13）、p-16（62.00%±10.85）和bcl-2（46.00%±42.47）高表达，而Ki-67（2.80%±2.55）、Rb（3.75%±4.55）、p-53（2.30%±0.10）、细胞周期蛋白A（0.70%±1.56）、B1（0.20%±0.34）和bax（2.65%±6.37）表达有限。细胞周期蛋白D1（8.60%±7.30）和p-21（6.40%±5.37）呈中度表达。与Spitz痣相比，黑色素瘤中bax（P = 0.001）、Ki-67（P < 0.0001）、Rb（P < 0.0001）、p-16（P < 0.0001）、细胞周期蛋白A（P < 0.0001）和细胞周期蛋白B1（P < 0.0001）的表达显著更高，而Spitz痣中p-27表达显著更高（P < 0.0001）。p-53也观察到有利于黑色素瘤的显著差异趋势（P = 0.002）。另一方面，bcl-2（P = 0.275）、p-21（P = 0.055）或细胞周期蛋白D1（P = 0.077）未检测到差异。与普通痣相比，Spitz痣显示出p-21（P = 0.008）和细胞周期蛋白D1（P = 0.006）更高表达的趋势，而p-16表达较低（P = 0.004）。