Pung O J, Kuhn R E
Department of Biology, Wake Forest University, Winston-Salem, NC 27109.
Int J Parasitol. 1991 Sep;21(5):503-10. doi: 10.1016/0020-7519(91)90054-b.
Adult, laboratory-bred squirrel monkeys (Saimiri sciureus) were infected with either Leishmania braziliensis braziliensis or L. b. panamensis and, 42 weeks later, they were challenge-infected with Trypanosoma cruzi. Another group of monkeys was infected with T. cruzi and challenged with L. b. braziliensis after 42 weeks. Immunoblotting was used to examine parasite antigens bound by antibodies in plasma obtained from the monkeys during the course of primary and challenge infections. During primary infections Leishmania-infected monkeys produced antibodies which bound to a number of Leishmania antigens, most notably a Leishmania antigen of 72 kDa, which were not recognized by antibodies produced by the monkeys given a primary infection of T. cruzi. These Leishmania-induced antibodies were no longer detectable 42 weeks after primary infections. However, when the Leishmania-infected monkeys were challenged with T. cruzi they once again produced antibodies capable of binding numerous Leishmania antigens, including the antigen of 72 kDa, which had not been recognized by antibodies produced by the monkeys with primary T. cruzi infections. A similar phenomenon was observed in T. cruzi-infected animals following Leishmania challenge.
成年实验室饲养的松鼠猴（松鼠猴属）感染了巴西利什曼原虫巴西亚种或巴拿马利什曼原虫，42周后，它们受到克氏锥虫的攻击感染。另一组猴子感染了克氏锥虫，并在42周后受到巴西利什曼原虫的攻击。免疫印迹法用于检测在初次感染和攻击感染过程中从猴子血浆中获得的抗体所结合的寄生虫抗原。在初次感染期间，感染利什曼原虫的猴子产生了与多种利什曼原虫抗原结合的抗体，最显著的是一种72 kDa的利什曼原虫抗原，而初次感染克氏锥虫的猴子产生的抗体则无法识别该抗原。初次感染42周后，这些利什曼原虫诱导产生的抗体不再能被检测到。然而，当感染利什曼原虫的猴子受到克氏锥虫攻击时，它们再次产生了能够结合多种利什曼原虫抗原的抗体，包括72 kDa的抗原，而初次感染克氏锥虫的猴子产生的抗体无法识别该抗原。在受到利什曼原虫攻击后，感染克氏锥虫的动物中也观察到了类似现象。
