一种从恶性疟原虫中纯化线粒体DNA的简单方法。
A simple method for the purification of mitochondrial DNA from Plasmodium falciparum.
作者信息
Flores M V, Stewart T S, O'Sullivan W J
机构信息
School of Biochemistry, University of New South Wales, Kensington, Australia.
出版信息
Int J Parasitol. 1991 Sep;21(5):605-8. doi: 10.1016/0020-7519(91)90067-h.
PMID:1743858
Abstract
Mitochondrial DNA (mtDNA) from Plasmodium falciparum was isolated by conventional differential centrifugation in an SS34 rotor, a simpler method than CsC1 centrifugation of total DNA as employed by other workers. The nature of the sample was verified by sequencing a polymerase chain reaction (PCR) product obtained using oligonucleotide primers derived from known malarial mtDNA sequence.
摘要
恶性疟原虫的线粒体DNA（mtDNA）通过在SS34转子中进行常规差速离心分离得到，这是一种比其他研究者采用的总DNA CsC1离心法更简单的方法。通过对使用源自已知疟疾mtDNA序列的寡核苷酸引物获得的聚合酶链反应（PCR）产物进行测序，验证了样品的性质。
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