Li Zhi-qing, Huang Yue-sheng, Yang Zong-cheng, Wang Jia-han
Department of Burns, Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China.
Zhonghua Shao Shang Za Zhi. 2006 Dec;22(6):448-51.
To investigate the effect of NF-kappaB activation on the early expression of proinflammatory cytokines in myocardium and early myocardial dysfunction in burn rats.
One hundred and seventy Wistar rats were enrolled in the study and randomly divided into three groups, i. e. control( C, n = 20, with isotonic saline solution) , burn ( B, n = 90, with isotonic solution after burns) and pyrrolidine dithiocarbamate (PDTC, n =60, with isotonic saline and 250 mg/kg PDTC after burns) groups. The rats in B and PDTC groups were inflicted with 35% TBSA full-thickness burns on the back. The activity of myocardial NF-kappaB was determined by electrophoretic mobility shift assay at 1 , 3, 6, 12,24 postburn hours (PBH), with expression of integral absorbance ( A ) value . The expression of myocardial tumor necrosis factor alpha ( TNF-alpha) mRNA was assessed by reverse transcription polymerase chain reaction( RT-PCR) and in situ hybridization at 6, 12 PBH, with expression ofA value. The left ventricular systolic pressure( LVSP) , the left ventricular end diastolic pressure (LVEDP) ,the maximum rate of rise of left ventricular pressure ( +/- dp/dt max) were also observed at 3, 6, 12,24 PBH. RESULTS The activity of myocardial NF-KB in B group was markedly increased at 1 PBH [ (20. 3+/-3. 4) x 104A ] ,which was obviously higher than that in C group (2. 2 +/- 0. 4) x 104A , P <0.01]. It peaked at 3 PBH, and was still evidently higher than that in C group at 24 PBH ( P <0. 01). The expression of TNF-alpha mRNA was obviously higher than that in C group at 3 PBH ( P < 0. 01) , peaking at 6 PBH, and it was mainly expressed in myocardium. The expression of LVSP and +/- dp/dt max were lower, but LVEDP was higher than that in C group during 3 -24 PBH ( P <0.01).
探讨核因子-κB（NF-κB）激活对烧伤大鼠心肌促炎细胞因子早期表达及早期心肌功能障碍的影响。
170只Wistar大鼠纳入研究，随机分为三组，即对照组（C组，n = 20，给予等渗盐水溶液）、烧伤组（B组，n = 90，烧伤后给予等渗溶液）和吡咯烷二硫代氨基甲酸盐（PDTC）组（n = 60，烧伤后给予等渗盐水和250 mg/kg PDTC）。B组和PDTC组大鼠背部造成35%体表面积的全层烧伤。于烧伤后1、3、6、12、24小时（PBH）采用电泳迁移率变动分析测定心肌NF-κB活性，并计算积分吸光度（A）值。于烧伤后6、12 PBH采用逆转录聚合酶链反应（RT-PCR）和原位杂交检测心肌肿瘤坏死因子-α（TNF-α）mRNA表达，并计算A值。于烧伤后3、6、12、24 PBH观察左心室收缩压（LVSP）、左心室舒张末期压力（LVEDP）、左心室压力最大上升速率（±dp/dt max）。结果：B组心肌NF-κB活性于烧伤后1小时明显升高[（20.3±3.4）×10⁴A]，明显高于C组（2.2±0.4）×10⁴A，P<0.01]。于烧伤后3小时达到峰值，至烧伤后24小时仍明显高于C组（P<0.01）。TNF-α mRNA表达于烧伤后3小时明显高于C组（P<0.01），于烧伤后6小时达到峰值，且主要在心肌中表达。在烧伤后3至24小时，B组LVSP和±dp/dt max低于C组，但LVEDP高于C组（P<0.01）。
