Harris Cory S, Burt Andrew J, Saleem Ammar, Le Phuong Mai, Martineau Louis C, Haddad Pierre S, Bennett Steffany A L, Arnason John T
Department of Biology and Center for Research in Biopharmaceuticals and Biotechnology, University of Ottawa, Ottawa, ON, Canada.
Phytochem Anal. 2007 Mar-Apr;18(2):161-9. doi: 10.1002/pca.970.
A method was developed for the analysis of Vaccinium angustifolium Ait. (Lowbush blueberry), which is a widely used natural health product, particularly for the treatment of diabetic symptoms. While the anthocyanin content of the fruit has been well characterized, the chemistry of the vegetative parts used in supportive therapy for diabetes has been largely ignored. Using a metabolomics-based approach for compound identification with an emphasis on phenolic metabolites, a single HPLC-PAD-APCI/ MS method was developed for the separation and quantitation of the major metabolites found in the 95% ethanol extracts of leaf, stem, root and fruit. The leaf extract contained high concentrations of chlorogenic acid (approximately 100 microg/mg extract) and a variety of quercetin glycosides that were also detected in the fruit and stem extracts. Flavan-3-ol monomers (+)-catechin and (-)-epicatechin were found in all plant parts but their procyanidin dimers were exclusively identified in the stem and root. The accuracy and precision of the presented method were corroborated by low intra- and inter-day variations in quantitative results in all plant part extracts. Further validation of the extraction and analytical protocols focused on identified compounds with reputed anti-diabetic activity, revealing recoveries greater than 80% and detection limits of 0.12-2.73 microg/mL.
开发了一种用于分析狭叶越橘（矮丛蓝莓）的方法，狭叶越橘是一种广泛使用的天然保健品，尤其用于治疗糖尿病症状。虽然该果实的花青素含量已得到充分表征，但在糖尿病支持性治疗中使用的营养器官的化学性质在很大程度上被忽视了。采用基于代谢组学的方法进行化合物鉴定，重点关注酚类代谢物，开发了一种单一的HPLC-PAD-APCI/MS方法，用于分离和定量叶、茎、根和果实95%乙醇提取物中发现的主要代谢物。叶提取物含有高浓度的绿原酸（约100μg/mg提取物）以及在果实和茎提取物中也检测到的多种槲皮素糖苷。黄烷-3-醇单体（+）-儿茶素和（-）-表儿茶素在所有植物部位均有发现，但其原花青素二聚体仅在茎和根中被鉴定出来。所有植物部位提取物定量结果的日内和日间低变化证实了所提出方法的准确性和精密度。对提取和分析方案的进一步验证集中在具有公认抗糖尿病活性的已鉴定化合物上，回收率大于80%，检测限为0.12 - 2.73μg/mL。
