Soma T, Shimada Y, Kawabe A, Kaganoi J, Kondo K, Imamura M, Uemoto S
Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Dis Esophagus. 2007;20(2):123-9. doi: 10.1111/j.1442-2050.2007.00657.x.
The synthesis of prostaglandin E2 (PGE2) requires cyclooxygenase (COX) and prostaglandin E synthase (PGES). There are two forms of PGES: cytosolic PGES (cPGES) and microsomal PGES (mPGES)-1. In this study, we investigated the effects of gastroesophageal reflux (GER) contents on PGES and COX-2 in esophageal cells. We incubated a human normal esophageal cell line, two esophageal squamous cell carcinoma (SCC) cell lines, and two esophageal adenocarcinoma (ADC) cell lines with GER contents. The production of PGE2 by these cells was assayed with an enzyme immunoassay kit. The protein expression of COX-2, cPGES, and mPGES-1 was confirmed by immunoblot analysis. The following results were obtained: GER contents induced the expression of COX-2 in all five cell lines. In normal esophageal cells, cPGES, but not mPGES-1, was detected in the cytosolic fraction. GER contents induced the expression of cPGES in the microsomal fraction. In SCC cells, cPGES was expressed in the cytosolic fraction, and mPGES-1 was expressed in the microsomal fraction. GER contents induced the expression of mPGES-1 in the microsomal fraction. In ADC cells, cPGES was expressed in both the cytosolic and microsomal fractions. GER contents induced the expression of both cPGES and mPGES-1 in the microsomal fraction. In conclusion, our results suggest that GER contents induce PGE2 production in esophageal cells. However, there are different isoforms of PGES in normal cells, SCC cells, and ADC cells.
前列腺素E2（PGE2）的合成需要环氧化酶（COX）和前列腺素E合酶（PGES）。PGES有两种形式：胞质型PGES（cPGES）和微粒体型PGES（mPGES）-1。在本研究中，我们调查了胃食管反流（GER）成分对食管细胞中PGES和COX-2的影响。我们用GER成分培养了一种人正常食管细胞系、两种食管鳞状细胞癌（SCC）细胞系和两种食管腺癌（ADC）细胞系。用酶免疫分析试剂盒检测这些细胞产生的PGE2。通过免疫印迹分析证实COX-2、cPGES和mPGES-1的蛋白表达。得到以下结果：GER成分诱导所有五种细胞系中COX-2的表达。在正常食管细胞的胞质部分检测到cPGES，但未检测到mPGES-1。GER成分诱导微粒体部分cPGES的表达。在SCC细胞中，cPGES在胞质部分表达，mPGES-1在微粒体部分表达。GER成分诱导微粒体部分mPGES-1的表达。在ADC细胞中，cPGES在胞质和微粒体部分均有表达。GER成分诱导微粒体部分cPGES和mPGES-1的表达。总之，我们的结果表明GER成分诱导食管细胞中PGE2的产生。然而，正常细胞、SCC细胞和ADC细胞中存在不同的PGES同工型。
