Takeda Yu, Kamida Tohru, Fujiki Minoru, Kobayashi Hidenori
Department of Neurosurgery, Oita University Faculty of Medicine, Oita, Japan.
Neurol Res. 2007 Mar;29(2):199-203. doi: 10.1179/016164107X188232.
Nogo-A, one of the axon regeneration inhibitors, has been shown to be up-regulated in both the experimental and human temporal lobe epilepsy. However, the role of Nogo-A in mossy fiber sprouting (MFS) relative to epileptogenesis is unknown. This work was designed to examine the relationship of the hippocampal Nogo-A protein expression with MFS during the development of amygdala kindling.
Using immunohistochemistry and neo-Timm's histological procedures, we evaluated the distribution and density of Nogo-A and Nogo-66 receptor (Ng-R) expression and MFS in the bilateral hippocampus of amygdala kindling rats.
Nogo-A expression in the ipsilateral hippocampus gradually increased with the development of kindling in the sector CA2-3. In contrast, no increased Nogo-A expression was observed in the contralateral hippocampus as the rats advanced to stage 5 kindled seizures. Furthermore, poorer Nogo-A and Nogo-66 receptor (Ng-R) expression were observed in the dentate granule cells as aberrant MFS occurred.
In amygdala kindling rats, generalized stage 5 seizures were not associated with increased Nogo-A expression in the contralateral hippocampus supporting the concept that seizures by themselves do not induce Nogo-A expression. Furthermore, in the ipsilateral hippocampus, the expression of Nogo-A relative to MSF suggests that this protein may partially control aberrant synaptic reorganization during epileptogenesis.
轴突再生抑制因子之一的Nogo-A已被证明在实验性和人类颞叶癫痫中均上调。然而，Nogo-A在苔藓纤维出芽（MFS）中相对于癫痫发生的作用尚不清楚。本研究旨在探讨杏仁核点燃过程中海马Nogo-A蛋白表达与MFS的关系。
采用免疫组织化学和改良的Timm组织学方法，评估杏仁核点燃大鼠双侧海马中Nogo-A和Nogo-66受体（Ng-R）表达的分布和密度以及MFS情况。
随着点燃在CA2-3区的发展，同侧海马中Nogo-A表达逐渐增加。相反，随着大鼠发展到第5期点燃发作，对侧海马中未观察到Nogo-A表达增加。此外，当出现异常MFS时，齿状颗粒细胞中Nogo-A和Nogo-66受体（Ng-R）表达较差。
在杏仁核点燃大鼠中，全身性第5期发作与对侧海马中Nogo-A表达增加无关，支持癫痫发作本身不会诱导Nogo-A表达这一观点。此外，在同侧海马中，Nogo-A相对于MSF的表达表明该蛋白可能在癫痫发生过程中部分控制异常的突触重组。
