伊朗多发性硬化症患者细胞因子基因多态性概况

Profile of cytokine gene polymorphisms in Iranian multiple sclerosis patients.

作者信息

Amirzargar A, Khosravi F, Dianat S, Hushmand F, Maryousef P, Foroushani A R, Lotfi J, Nikbin B

机构信息

Immunogenetic Laboratory, Department of Immunology, Medical School, Tehran University of Medical Sciences, Tehran, Iran.

出版信息

Mult Scler. 2007 Mar;13(2):253-5. doi: 10.1177/1352458506070237. Epub 2007 Jan 29.

DOI:10.1177/1352458506070237

PMID:17439892

Abstract

BACKGROUND

Cytokine gene polymorphisms have been extensively studied in association with different diseases. The role of cytokine gene polymorphisms in multiple sclerosis (MS), as a chronic immune-mediated neurodegenerative disease, has been previously reported.

MATERIALS AND METHODS

DNA samples were collected from 44 patients with relapsing-remitting multiple sclerosis (RRMS) and 140 unrelated healthy subjects. All participants in this study were matched for ethnicity. Cytokine gene SNPs were determined using the PCR-SSP method.

RESULTS

and discussion We found no significant differences between MS patients and controls in most of the studied cytokine genes. Remarkable results were obtained for IL-2 GG-330 genotype (P =0.06), IL-6 C-174 allele (P =0.06), CG and GG genotypes (P <0.001), and GG (P =0.02) and CG (P <0.001) haplotypes, and TNF-alpha A-238 allele (P <0.001), GG (P =0.003) and GA (P <0.001) haplotypes. These results suggest that polymorphic variations of these pro-inflammatory cytokines play an important role in susceptibility to MS.

摘要

背景

细胞因子基因多态性已被广泛研究与不同疾病的关联。细胞因子基因多态性在作为一种慢性免疫介导的神经退行性疾病的多发性硬化症（MS）中的作用此前已有报道。

材料与方法

从44例复发缓解型多发性硬化症（RRMS）患者和140名无亲缘关系的健康受试者中收集DNA样本。本研究的所有参与者在种族上相匹配。使用PCR - SSP方法测定细胞因子基因单核苷酸多态性。

结果与讨论

我们发现，在大多数研究的细胞因子基因中，MS患者与对照组之间无显著差异。在IL - 2 GG - 330基因型（P = 0.06）、IL - 6 C - 174等位基因（P = 0.06）、CG和GG基因型（P < 0.001）以及GG（P = 0.02）和CG（P < 0.001）单倍型方面获得了显著结果，以及肿瘤坏死因子 - α A - 238等位基因（P < 0.001）、GG（P = 0.003）和GA（P < 0.001）单倍型。这些结果表明，这些促炎细胞因子的多态性变异在MS易感性中起重要作用。