Haga J A, Riley W J, Kao K J
Department of Pathology and Laboratory Medicine, University of Florida College of Medicine, Gainesville 32610-0275.
Hum Immunol. 1991 Oct;32(2):79-84. doi: 10.1016/0198-8859(91)90103-g.
To understand the complexity of plasma HLA antigens, the distribution of different molecular weight forms of class I HLA in plasma was investigated in 44 HLA-phenotyped and unrelated individuals. Plasma class I HLA were immunoprecipitated by using the W6/32 anti-HLA monoclonal antibody, separated by SDS-polyacrylamide gel electrophoresis and characterized by immunoblotting with the HC-10 monoclonal antibody. Four different forms of HLA heavy chains (HLA-HC) with relative molecular masses of 44, 39, 36, and 34 kd were detected. Plasma samples from all individuals contained 44 and 36 kd HLA-HC, but varied as to the presence of 39 and 34 kd HLA-HC. Eighteen percent of the individuals did not have any detectable class I HLA with 39-kd heavy chains in their plasma and 61% did not have plasma class I HLA with 34-kd heavy chains. Thus, four different distribution patterns were identified for plasma class I HLA among all individuals included in our study. The distribution patterns in four different individuals were evaluated quarterly and remained unchanged during 1 year follow-up. A significant association of absence of 39-kd plasma class I HLA-HC with female gender (p less than 0.05) and HLA-B7 phenotype (p less than 0.00015) was also found. Further pedigree analyses of four families of HLA-B7-positive and 39-kd HLA-HC-negative probands indicated that genetic factor(s) other than those associated with HLA-B7 allele and female gender is involved in regulating the expression of the plasma class I HLA with 39-kd heavy chains.
为了解血浆HLA抗原的复杂性，我们对44名HLA表型不同且无亲缘关系的个体进行了研究，以调查血浆中不同分子量形式的I类HLA的分布情况。使用W6/32抗HLA单克隆抗体对血浆I类HLA进行免疫沉淀，通过SDS-聚丙烯酰胺凝胶电泳分离，并使用HC-10单克隆抗体进行免疫印迹鉴定。检测到四种相对分子量分别为44、39、36和34kd的不同形式的HLA重链（HLA-HC）。所有个体的血浆样本均含有44kd和36kd的HLA-HC，但39kd和34kd的HLA-HC的存在情况各不相同。18%的个体血浆中未检测到任何39kd重链的I类HLA，61%的个体血浆中未检测到34kd重链的I类HLA。因此，在我们研究的所有个体中，确定了血浆I类HLA的四种不同分布模式。对四个不同个体的分布模式每季度进行评估，在1年的随访期间保持不变。还发现血浆中缺乏39kd的I类HLA-HC与女性性别（p<0.05）和HLA-B7表型（p<0.00015）之间存在显著关联。对四个HLA-B7阳性且39kd HLA-HC阴性先证者家族的进一步系谱分析表明，除了与HLA-B7等位基因和女性性别相关的基因因素外，还有其他基因因素参与调节血浆中39kd重链的I类HLA的表达。
