Sanz Ernesto, Peñas Lucas, Lequerica Juan L
Valencia, Spain From the Instituto de Biomedicina de Valencia, Spanish Council for Scientific Research, and Department of Pathology, Hospital Universitario "La Fe."
Plast Reconstr Surg. 2007 May;119(6):1707-1713. doi: 10.1097/01.prs.0000258849.76630.d2.
The availability of generated cartilage de novo is one of the needs of reconstructive surgery. In this study, the authors constructed a matrix formed by autologous immobilized chondrocytes using collagen gel as a scaffold. Furthermore, the ability of these matrices to engraft and generate new cartilage was examined.
Biopsy specimens of elastic cartilage were surgically obtained from the ears of eight New Zealand White rabbits. After collagenase II digestion of cartilage, chondrocytes were isolated and propagated in culture medium. Chondrocytes were immobilized into bovine collagen lattices and implanted, replacing pieces of removed native cartilage. Five weeks after implantation, the rabbits were killed and the ears were examined macroscopically and analyzed by means of histochemical methods.
The results show the formation of new cartilage from implanted lattices with chondrocytes. Gross analysis of the ears shows similarities in appearance, consistency, texture, and histology between native and new cartilage. Fluorescence of the nucleus from bisbenzimide-labeled chondrocytes was detected in newly formed tissue, pointing out its in vitro culture origin. No signs of an inflammatory reaction attributable to implants were found in either the control or the chondrocyte lattices.
The authors suggest that this approach is of value for future clinical use.
从头生成软骨是重建手术的需求之一。在本研究中，作者使用胶原凝胶作为支架构建了由自体固定化软骨细胞形成的基质。此外，还检测了这些基质植入并生成新软骨的能力。
通过手术从八只新西兰白兔的耳朵获取弹性软骨活检标本。软骨经Ⅱ型胶原酶消化后，分离软骨细胞并在培养基中扩增。将软骨细胞固定于牛胶原网格中并植入，替换部分切除的天然软骨。植入后5周，处死兔子，对耳朵进行宏观检查并采用组织化学方法分析。
结果显示植入含软骨细胞的网格后形成了新软骨。耳朵的大体分析显示天然软骨和新软骨在外观、质地、纹理和组织学方面相似。在新形成的组织中检测到双苯甲酰亚胺标记的软骨细胞核的荧光，表明其来源于体外培养。在对照组或软骨细胞网格中均未发现植入物引起的炎症反应迹象。
作者认为这种方法对未来临床应用具有价值。
