Poksinski Michal, Arwin Hans
Department of Physics, Biology and Chemistry, Laboratory of Applied Optics, Linköping University, Linköping, Sweden.
Opt Lett. 2007 May 15;32(10):1308-10. doi: 10.1364/ol.32.001308.
Total internal reflection ellipsometry (TIRE) is used to study adsorption of human serum albumin and fibrinogen on thin gold films. TIRE shows very high sensitivity for protein monolayers adsorbed on metal surfaces when surface plasmon resonance effects are utilized. The measured data, expressed in ellipsometric angles psi and D are of several orders of magnitude larger in comparison with those from similar experiments performed with traditional ellipsometry. TIRE in spectral mode opens a new path for precise studies of organic layers adsorbed on metal surfaces, with a potential for resolving the adsorbed layer microstructure.
全内反射椭圆偏振光谱法（TIRE）用于研究人血清白蛋白和纤维蛋白原在金薄膜上的吸附。当利用表面等离子体共振效应时，TIRE对吸附在金属表面的蛋白质单层具有非常高的灵敏度。与传统椭圆偏振光谱法进行的类似实验相比，以椭圆偏振角ψ和Δ表示的测量数据要大几个数量级。光谱模式下的TIRE为精确研究吸附在金属表面的有机层开辟了一条新途径，具有解析吸附层微观结构的潜力。
