[外源性神经节苷脂GM1在神经干细胞增殖与分化中的作用]
[Role of extrinsic ganglioside GM1 in proliferation and differentiation of neural stem cells].
作者信息
Duan Jian-Gang, Xiang Tao, Chen Hong, Liu Ming
机构信息
Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2007 Mar;38(2):260-3.
OBJECTIVE
To study the possible role of ganglioside GM1 in proliferation and differentiation of neural stem cells (NSCs) of rats in vitro.
METHODS
(1) Fetal rats were employed to this study. The primary cells isolated from the cerebral cortex and hippocampus were cultured with serum-free incubation technique and then the cellular differentiation were induced with serum. (2) The proliferation and differentiation features of NSCs were observed under phase contrast microscope after the GM1 with different concentrations were added into NSCs' and serum-free DMEM/F12 culture media.
RESULTS
(1) Comparing with control, no significant proliferation of NSCs was coming to pass when the GM1 with low concentration was added into the cell culture medium; however, the size of NSCs clones gradually decreased and the cells were dying when the GM1 with high concentration was added into NSCs' culture medium. (2) NSCs, cultured in DMEM/F12 culture medium only containing GM1, neither proliferated nor differentiated, but were dying soon.
CONCLUSION
GM1 of low concentration (12.5 microg/mL) has no significant influence on proliferation of NSCs, but GM1 of higher concentration can inhibit the NSCs' proliferation in culture medium.
目的
研究神经节苷脂GM1在体外对大鼠神经干细胞（NSCs）增殖和分化的可能作用。
方法
（1）本研究采用胎鼠。从大脑皮质和海马分离的原代细胞采用无血清培养技术培养，然后用血清诱导细胞分化。（2）将不同浓度的GM1加入NSCs和无血清DMEM/F12培养基中，在相差显微镜下观察NSCs的增殖和分化特征。
结果
（1）与对照组相比，向细胞培养基中加入低浓度GM1时，NSCs无明显增殖；然而，向NSCs培养基中加入高浓度GM1时，NSCs克隆的大小逐渐减小，细胞死亡。（2）仅在含有GM1的DMEM/F12培养基中培养的NSCs既不增殖也不分化，但很快死亡。
结论
低浓度（12.5μg/mL）的GM1对NSCs的增殖无明显影响，但高浓度的GM1可抑制培养基中NSCs的增殖。
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