[叶酸对体外神经干细胞增殖与分化的影响]
[Effects of folate on proliferation and differentiation of neural stem cells in vitro].
作者信息
Ji Changzhen, Huang Guowei, Zhang Wenzhi, Su Xin
机构信息
School of Public Health, Tianjin Medical University, Tianjin 300070, China.
出版信息
Wei Sheng Yan Jiu. 2007 Sep;36(5):578-80.
OBJECTIVE
To explore the protective effect of folic acid on proliferation and differentiation of neural stem cells in vitro.
METHODS
On the 14th day of gestation, NSCs were obtained. MTT method was used to detect the proliferation of NSCs cultured with folic acid of different concentrations and time. After 6 days, the NSCs purified were placed into culture dishes of fetus bovine serum. On the seventh day of differentiation, double-tabeling immunofluorescence with antibody Nestin and BrdU was taken to calculate the number of NSCs by 8 fields of vision chosen randomly .
RESULTS
Comparing to control group, NSCs of folate group was increase and better, and significantly (P < 0.05).
CONCLUSION
Folic acid may suppress proliferation and differentiation of NSCs efficiently.
目的
探讨叶酸对体外培养神经干细胞增殖与分化的保护作用。
方法
取妊娠第14天的神经干细胞。采用MTT法检测不同浓度叶酸及不同时间培养的神经干细胞的增殖情况。6天后，将纯化的神经干细胞接种于含胎牛血清的培养皿中。分化第7天，采用巢蛋白和BrdU抗体进行双标免疫荧光，随机选取8个视野计算神经干细胞数量。
结果
与对照组相比，叶酸组神经干细胞数量增多且状态更佳，差异有统计学意义（P<0.05）。
结论
叶酸可有效抑制神经干细胞的增殖与分化。
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