采用柱前衍生化法的液相色谱法测定氰胺化钙。

Liquid chromatographic method for the determination of calcium cyanamide using pre-column derivatization.

作者信息

Chen S, Ocampo A P, Kucera P J

机构信息

Analytical Development, Lederle Laboratories, Pearl River, NY 10965.

出版信息

J Chromatogr. 1991 Sep 27;558(1):141-6. doi: 10.1016/0021-9673(91)80119-2.

DOI:10.1016/0021-9673(91)80119-2

PMID:1744211

Abstract

A specific and stability-indicating high-performance liquid chromatographic (HPLC) method has been developed for the analysis of calcium cyanamide in bulk material and dosage form. Calcium cyanamide in samples was converted into dansyl cyanamide. A muBondapak C18 column was employed for HPLC with 0.01 M sodium phosphate (pH 6.3)-acetonitrile (75:25, v/v) as the mobile phase. The proposed HPLC method was validated for linearity, specificity, accuracy and reproducibility.

摘要

已开发出一种用于分析原料药和剂型中氰胺化钙的特异性和稳定性指示高效液相色谱（HPLC）方法。样品中的氰胺化钙被转化为丹磺酰氰胺。采用μBondapak C18柱进行HPLC分析，以0.01 M磷酸钠（pH 6.3）-乙腈（75:25，v/v）作为流动相。所提出的HPLC方法在线性、特异性、准确性和重现性方面进行了验证。