Luo Qing-li, He Wei-min, Xia Qing-jie, Cao Gui-qun, Yan Nai-hong, Wang Ling, Zhang Fa-qiang
Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhonghua Yan Ke Za Zhi. 2007 Jan;43(1):40-3.
To evaluate the expression of glucocorticoid receptor alpha (GRalpha) and beta (GRbeta) messenger RNA (mRNA) in orbital tissues from thyroid associated ophthalmopathy (TAO).
Samples of extraocular muscle and orbital fat were obtained from 17 patients with TAO and 10 healthy individuals. Total RNA was extracted and reversely transcripted into cDNA. The expression of GRalpha and GRbeta mRNA was detected by means of fluorescent quantitative polymerase chain reaction (PCR).
Expression of GRalpha mRNA was much higher than GRbeta mRNA in all extraocular muscle and orbital fat biopsies. The relative copy of GRalpha was 40.15 +/- 11.37 in TAO patients and 20.64 +/- 7.07 in the controls. GRalpha: GRbeta mRNA ratio of these two groups was 77.76 +/- 18.77 and 148.34 +/- 23.86, respectively. There was significant difference between these two groups (P < 0.05). No significant difference was noted between extraocular muscle and orbital fat biopsies, between glucocorticoid-treated and non-treated patients or among hyperthyroidism, hypothyroidism and euthyroidism (P > 0.05).
The increased expression of GRalpha mRNA and decreased GRalpha: GRbeta ratio in orbital tissues may play an important role in the pathogenesis of TAO and the effects of glucocorticoid treatment.
评估糖皮质激素受体α（GRα）和β（GRβ）信使核糖核酸（mRNA）在甲状腺相关眼病（TAO）眼眶组织中的表达。
从17例TAO患者和10名健康个体获取眼外肌和眼眶脂肪样本。提取总RNA并逆转录为互补脱氧核糖核酸（cDNA）。通过荧光定量聚合酶链反应（PCR）检测GRα和GRβ mRNA的表达。
在所有眼外肌和眼眶脂肪活检样本中，GRα mRNA的表达远高于GRβ mRNA。TAO患者中GRα的相对拷贝数为40.15±11.37，对照组为20.64±7.07。这两组的GRα:GRβ mRNA比值分别为77.76±18.77和148.34±23.86。两组间差异有统计学意义（P<0.05）。眼外肌和眼眶脂肪活检样本之间、糖皮质激素治疗和未治疗患者之间或甲状腺功能亢进、甲状腺功能减退和甲状腺功能正常患者之间差异均无统计学意义（P>0.05）。
眼眶组织中GRα mRNA表达增加及GRα:GRβ比值降低可能在TAO发病机制及糖皮质激素治疗效果中起重要作用。
