Rocha-Peña M A, Lee R F
University of Florida, Citrus Research and Education Center, Lake Alfred.
J Virol Methods. 1991 Oct;34(3):311-31. doi: 10.1016/0166-0934(91)90109-d.
Citrus tristeza virus (CTV) is the most economically important virus disease of citrus. In the last ten years, remarkable progress has been achieved in the development and improvement of new serological methods for CTV detection so that serology has become a dependable tool for many research, extension and regulatory purposes worldwide. CTV-specific polyclonal antisera and monoclonal antibodies have been developed in different research laboratories and used extensively in a wide range of different studies. This review describes the diverse serological methods developed for CTV detection and analyzes the advantages, disadvantages, relative sensitivity, applications, and present status of each method.
柑橘衰退病毒(CTV)是柑橘类最具经济重要性的病毒病害。在过去十年中,用于CTV检测的新血清学方法的开发和改进取得了显著进展,因此血清学已成为全球许多研究、推广和监管目的的可靠工具。不同的研究实验室已开发出CTV特异性多克隆抗血清和单克隆抗体,并广泛应用于各种不同的研究中。本综述描述了为CTV检测而开发的多种血清学方法,并分析了每种方法的优缺点、相对灵敏度、应用及现状。