Nong T, Saito K, Blair L, Tarsitani C, Lee J-H
One Lambda, Inc., Canoga Park, CA 91303-2801, USA.
Tissue Antigens. 2007 Apr;69 Suppl 1:92-5. doi: 10.1111/j.1399-0039.2006.762_3.x.
Recent studies suggest that killer cell immunoglobulin-like receptors (KIR) genotyping may be relevant to bone marrow/stem cell transplantation. The objective of this research was to develop a bead-based reverse sequence-specific oligonucleotide DNA hybridization assay for KIR genotyping using the Luminex platform. The oligonucleotide probes were designed to recognize 14 currently known KIR genes and two pseudogenes, as well as null alleles. A unique probe design was used to allow detection of two cis-polymorphic regions for typing of KIR2DS4, because the functioning alleles cannot readily be assigned by conventional probe detection systems. Assay performance was validated using DNA samples previously typed by the 13th International Histocompatibility Working Group with 100% concordant results.
近期研究表明，杀伤细胞免疫球蛋白样受体（KIR）基因分型可能与骨髓/干细胞移植相关。本研究的目的是开发一种基于微珠的反向序列特异性寡核苷酸DNA杂交检测方法，用于利用Luminex平台进行KIR基因分型。寡核苷酸探针被设计用于识别14个目前已知的KIR基因、两个假基因以及无效等位基因。采用了独特的探针设计以检测KIR2DS4分型的两个顺式多态性区域，因为传统探针检测系统难以确定功能性等位基因。使用第13届国际组织相容性工作组先前分型的DNA样本对检测性能进行验证，结果一致性达100%。
