Saidman S L
Histocompatibility Laboratory, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
Transplant Proc. 2007 Apr;39(3):673-5. doi: 10.1016/j.transproceed.2007.01.053.
HLA antibodies can develop for many different reasons and are often present in the serum of patients awaiting renal transplantation. These antibodies can limit the patient's ability to find a compatible donor and are a risk factor for poor graft outcome. Detection of HLA antibodies is important for identifying compatible donors, assessing the pretransplantation risk for rejection, and diagnosing humoral rejection posttransplantation. The results of antibody screens are also useful when interpreting crossmatch results. Assays used for detecting panel-reactive and donor-specific HLA antibodies, including cytotoxicity, flow cytometry, and solid phase assays, are described in this review.
HLA抗体可因多种不同原因产生，且常存在于等待肾移植患者的血清中。这些抗体可能会限制患者找到相容供体的能力，并且是移植效果不佳的一个风险因素。检测HLA抗体对于识别相容供体、评估移植前排斥反应风险以及诊断移植后体液性排斥反应均很重要。在解释交叉配型结果时，抗体筛查结果也很有用。本文综述了用于检测群体反应性和供体特异性HLA抗体的检测方法，包括细胞毒性试验、流式细胞术和固相检测法。
