Zerani M, Gobbetti A
Department of Molecular, Cellular and Animal Biology, University of Camerino, Italy.
J Endocrinol. 1991 Nov;131(2):295-302. doi: 10.1677/joe.0.1310295.
The effects of beta-endorphin and its receptor antagonist, naloxone, on corticosterone and cortisol production in male and female Triturus carnifex were studied in vivo and in vitro. In the in-vivo experiment, the animals were injected s.c. with beta-endorphin and/or naloxone, and killed after 15, 30, 90 and 360 min. In the in-vitro experiment, interrenal tissues, with and without added pituitary, were incubated with beta-endorphin and/or naloxone for 15, 30, 60 and 120 min. The data obtained in vivo and in vitro from males and females were in agreement. Treatment with beta-endorphin caused a significant decrease in corticosterone and cortisol release, while naloxone induced an increase in the two corticosteroids at the same times as the decrease caused by beta-endorphin. The combined beta-endorphin plus naloxone treatment did not change corticosterone and cortisol levels. These results suggest that, in Triturus carnifex, opioids are involved in the regulation of the hypothalamo-pituitary-interrenal axis. In particular, the in-vitro results indicate a direct effect of opioids on interrenal steroidogenesis.
在体内和体外研究了β-内啡肽及其受体拮抗剂纳洛酮对雄性和雌性意大利真螈皮质酮和皮质醇分泌的影响。在体内实验中，给动物皮下注射β-内啡肽和/或纳洛酮，并在15、30、90和360分钟后处死。在体外实验中，将添加和未添加垂体的肾间组织与β-内啡肽和/或纳洛酮一起孵育15、30、60和120分钟。从雄性和雌性动物体内和体外获得的数据是一致的。用β-内啡肽处理导致皮质酮和皮质醇释放显著减少，而纳洛酮在β-内啡肽引起减少的同时诱导这两种皮质类固醇增加。β-内啡肽加纳洛酮联合处理并未改变皮质酮和皮质醇水平。这些结果表明，在意大利真螈中，阿片类物质参与下丘脑-垂体-肾间轴的调节。特别是，体外实验结果表明阿片类物质对肾间类固醇生成有直接作用。
