γ-氨基丁酸A受体相关氯离子通道参与γ-氨基丁酸A受体激动剂蝇蕈醇诱导的外周镇痛作用。

Involvement of GABA A receptor-associated chloride channels in the peripheral antinociceptive effect induced by GABA A receptor agonist muscimol.

作者信息

Reis Glaucia, Pacheco Daniela, Francischi Janetti, Castro Maria, Perez Andréa, Duarte Igor

机构信息

Department of Pharmacology, Institute of Biological Sciences, Federal University of Minas Gerais, Av. Antônio Carlos 6627, 31270-100 Belo Horizonte, MG, Brazil.

出版信息

Eur J Pharmacol. 2007 Jun 14;564(1-3):112-5. doi: 10.1016/j.ejphar.2007.02.043. Epub 2007 Mar 3.

DOI:10.1016/j.ejphar.2007.02.043

PMID:17445797

Abstract

The effect of chloride and potassium channel blockers on the antinociception induced by GABA(A) receptor agonist muscimol was investigated using the paw pressure test. Muscimol (1, 2, 4, 8 ng/paw) elicited a peripheral antinociceptive effect that was antagonized by bicuculline (10, 20, 40, 80 ng/paw), suggesting a specific effect. The muscimol effect was reverted by the chloride channel coupled GABA(A) receptor blocker, picrotoxin (0.4, 0.6, 0.8, 2 microg/paw). Potassium channel blockers did not modify the peripheral antinociception induced by muscimol. This study provides evidence that the peripheral antinociceptive effect of muscimol results from the activation of GABA(A) receptor-associated chloride channels.

摘要

使用 paw 压力测试研究了氯离子和钾离子通道阻滞剂对 GABA(A) 受体激动剂蝇蕈醇诱导的抗伤害感受的影响。蝇蕈醇（1、2、4、8 ng/爪）引发了外周抗伤害感受作用，该作用被荷包牡丹碱（10、20、40、80 ng/爪）拮抗，提示有特异性作用。蝇蕈醇的作用被氯离子通道偶联的 GABA(A) 受体阻滞剂印防己毒素（0.4、0.6、0.8、2 μg/爪）逆转。钾离子通道阻滞剂未改变蝇蕈醇诱导的外周抗伤害感受。本研究提供了证据表明蝇蕈醇的外周抗伤害感受作用源于 GABA(A) 受体相关氯离子通道的激活。