Rubin W, Aliasgharpour A A
Anat Rec. 1976 Feb;184(2):251-64. doi: 10.1002/ar.1091840210.
Pieces of rat and human gastric mucosa were fixed in glutaraldehyde and paraformaldehyde and were processed by the methods of Wachstein-Meisel and of Ernst for the cytochemical demonstration of adenosine triphosphatase (ATPase) and p-nitrophenylphosphatase (NPPase) respectively. Electron microscopic examination of reacted sections revealed the ATPase reaction to be localized to the plasmalemma and to mitochondrial cristae and the NPPase reaction to the plasmalemma. The tubulovesicles were unreactive. By thus demonstrating a cytochemical difference between the plasmalemma and tubulovesicle membrane, this study suggests that the tubulovesicles do not represent an extension of or reserve of mature plasmalemma. If the tubulovesicles represent a reserve of membrane that can be rapidly utilized to expand the plasmalemma, then these studies suggest that additional membrane constituents are added, activated, or unmasked in the process.
将大鼠和人类胃黏膜组织块用戊二醛和多聚甲醛固定，分别采用瓦施泰因 - 迈泽尔法和恩斯特法进行处理，以分别对三磷酸腺苷酶（ATP酶）和对硝基苯磷酸酶（NPP酶）进行细胞化学显示。对反应切片进行电子显微镜检查发现，ATP酶反应定位于质膜和线粒体嵴，而NPP酶反应定位于质膜。微管泡无反应。通过如此证明质膜和微管泡膜之间的细胞化学差异，本研究表明微管泡并不代表成熟质膜的延伸或储备。如果微管泡代表可迅速用于扩展质膜的膜储备，那么这些研究表明在此过程中会添加、激活或暴露其他膜成分。
