Bauer Katja, Schrader Christina, Suess Jochen, Wutzler Peter, Schmidtke Michaela
Institute of Virology and Antiviral Therapy, Medical Centre of the Friedrich Schiller University Jena, Hans Knoell Str. 2, PF, D-07740 Jena, Germany.
Antiviral Res. 2007 Sep;75(3):219-26. doi: 10.1016/j.antiviral.2007.03.007. Epub 2007 Apr 3.
As an intermediate host of avian and human influenza A viruses (FLUAV) pigs may play a potential role in interspecies virus transmission and reassortment of viral genes including those conferring antiviral drug resistance. Porcine FLUAV isolated in Germany between 1989 and 2001 contains mutations in the M2 gene inducing amantadine resistance. No data exist on neuraminidase inhibitor (NAI) susceptibility of these porcine FLUAV. We studied the antiviral activity of NAI against seven selected H3N2 FLUAV isolated from pigs in Germany between 1982 and 1999. All isolates were susceptible towards oseltamivir and zanamivir in neuraminidase enzyme-inhibition assays. Both compounds inhibited virus spreading and reduced the virus yields and plaque size at low concentrations. Higher concentrations were necessary to reduce the plaque number. Two isolates that differed in glycosylation pattern of viral hemagglutinin (HA) showed markedly reduced drug susceptibility in cell culture-based assays.
作为甲型禽流感病毒和人流感病毒（FLUAV）的中间宿主，猪可能在病毒跨物种传播以及包括赋予抗病毒耐药性的基因在内的病毒基因重配中发挥潜在作用。1989年至2001年间在德国分离出的猪流感病毒（FLUAV）在M2基因中存在诱导金刚烷胺耐药性的突变。关于这些猪流感病毒（FLUAV）对神经氨酸酶抑制剂（NAI）的敏感性尚无数据。我们研究了NAI对1982年至1999年间从德国猪中分离出的7种选定的H3N2流感病毒（FLUAV）的抗病毒活性。在神经氨酸酶抑制试验中，所有分离株对奥司他韦和扎那米韦均敏感。两种化合物在低浓度时均能抑制病毒传播，并降低病毒产量和蚀斑大小。需要更高的浓度才能减少蚀斑数量。两种病毒血凝素（HA）糖基化模式不同的分离株在基于细胞培养的试验中显示出明显降低的药物敏感性。
