Sabumon P C
Environmental and Water Resources Engineering Division, Department of Civil Engineering, IIT Madras, Chennai 600036, India.
J Hazard Mater. 2007 Oct 1;149(1):49-59. doi: 10.1016/j.jhazmat.2007.03.052. Epub 2007 Mar 24.
This study describes the feasibility of anaerobic ammonia removal process in presence of organic matter. Different sources of biomass collected from diverse eco-systems containing ammonia and organic matter (OM) were screened for potential anaerobic ammonia removal. Sequential batch studies confirmed the possibility of anaerobic ammonia removal in presence of OM, but ammonia was oxidized anoxically to nitrate (at oxidation reduction potential; ORP=-248+/-25 mV) by an unknown mechanism unlike in the reported anammox process. The oxygen required for oxidation of ammonia might have been generated through catalase enzymatic activity of facultative anaerobes in mixed culture. The oxygen generation possibility by catalase enzyme route was demonstrated. Among the inorganic electron acceptors (NO(2)(-), NO(3)(-) and SO(4)(2-)) studied, NO(2)(-) was found to be most effective in total nitrogen removal. Denitrification by the developed culture was much effective and faster compared to ammonia oxidation. The results of this study show that anaerobic ammonia removal is feasible in presence of OM. The novel nitrogen removal route is hypothesized as enzymatic anoxic oxidation of NH(4)(+) to NO(3)(-), followed by denitrification via autotrophic and/or heterotrophic routes. The results of batch study were confirmed in continuous reactor operation.
本研究描述了在存在有机物的情况下厌氧氨去除过程的可行性。对从含有氨和有机物（OM）的不同生态系统中收集的不同来源的生物质进行筛选，以寻找潜在的厌氧氨去除能力。序批式研究证实了在存在OM的情况下厌氧氨去除的可能性，但与报道的厌氧氨氧化过程不同，氨通过未知机制在缺氧条件下被氧化为硝酸盐（在氧化还原电位；ORP = -248±25 mV）。氨氧化所需的氧气可能是通过混合培养中兼性厌氧菌的过氧化氢酶活性产生的。通过过氧化氢酶途径产生氧气的可能性得到了证实。在所研究的无机电子受体（NO₂⁻、NO₃⁻和SO₄²⁻）中，发现NO₂⁻在总氮去除方面最有效。与氨氧化相比，所培养的菌群进行的反硝化作用更有效且更快。本研究结果表明，在存在OM的情况下厌氧氨去除是可行的。推测新的氮去除途径为NH₄⁺通过酶促缺氧氧化为NO₃⁻，随后通过自养和/或异养途径进行反硝化。序批式研究的结果在连续反应器运行中得到了证实。
