Ohtaka-Maruyama Chiaki, Miwa Akiko, Kawano Hitoshi, Kasai Masataka, Okado Haruo
Department of Molecular Physiology, Tokyo Metropolitan Institute for Neuroscience, Tokyo 183-8526, Japan.
J Comp Neurol. 2007 Jun 20;502(6):1098-108. doi: 10.1002/cne.21350.
RP58, a novel zinc finger protein containing a POZ domain, is a sequence-specific transcriptional repressor. To understand the role of this protein, we examined RP58 gene expression in the developing mouse brain by quantitative polymerase chain reaction (PCR) and in situ hybridization. RP58 mRNA expression was detected at embryonic day (E) 10 in the neuroepithelium, and subsequently in the ventricular zones of the cerebral cortex in the E12 embryo. Strong expression was observed in the preplate in the cerebral cortex from this stage onward. High levels of expression continued to be detected in the cortical plate and subventricular zone of the neocortex, hippocampus, and parts of the amygdala, but not in the thalamus or striatum. These results suggest that RP58 plays a crucial role in neuronal proliferation, migration, and differentiation in the developing cerebral cortex. RP58 is also expressed in the adult mouse neocortex, hippocampus, parts of the amygdala, and granule cells in the cerebellum. Double in situ hybridization using GAD67 or VGLUT1 probes revealed that RP58 is expressed in glutamatergic excitatory neurons.
RP58是一种含有POZ结构域的新型锌指蛋白，是一种序列特异性转录抑制因子。为了解该蛋白的作用，我们通过定量聚合酶链反应（PCR）和原位杂交技术检测了发育中小鼠大脑中RP58基因的表达情况。在胚胎第10天（E10）的神经上皮中检测到RP58 mRNA表达，随后在E12胚胎大脑皮质的脑室区也检测到该表达。从这个阶段开始，在大脑皮质的前板中观察到强表达。在新皮质、海马体和部分杏仁核的皮质板和脑室下区持续检测到高水平表达，但在丘脑或纹状体中未检测到。这些结果表明，RP58在发育中的大脑皮质神经元增殖、迁移和分化中起关键作用。RP58在成年小鼠的新皮质、海马体、部分杏仁核和小脑颗粒细胞中也有表达。使用GAD67或VGLUT1探针进行的双重原位杂交显示，RP58在谷氨酸能兴奋性神经元中表达。
