Rouet Francois, Rouzioux Christine
Laboratoire de Virologie, Centre Muraz, Bobo-Dioulasso, Burkina Faso, West Africa.
Clin Lab. 2007;53(3-4):135-48.
There is an urgent need for low-cost, simple, and accurate human immunodeficiency virus type 1 (HIV-1) viral load monitoring technologies in resource-limited settings, particularly at the time of the scaling-up of first and second-line highly active antiretroviral therapies. This review describes the main characteristics and advantages/ disadvantages of three alternative HIV-1 viral load methods currently evaluated and used in developing countries, i.e., the Ultra p24 antigen assay, the ExaVir Load reverse transcriptase activity test, and 'home-made' real-time PCR HIV-1 RNA techniques. This review discusses clinical results obtained with these three technologies in terms of correlation with commercial HIV-1 RNA assays, the impact of HIV-1 genetic diversity on quantification, as well as their usefulness for both the early diagnosis of pediatric HIV-1 infection and monitoring of highly active antiretroviral therapy efficiency. In addition, different strategies for HIV-1 viral load monitoring are discussed according to laboratory facilities in resource-constrained settings.
在资源有限的环境中，尤其是在扩大一线和二线高效抗逆转录病毒疗法规模之际，迫切需要低成本、简单且准确的1型人类免疫缺陷病毒（HIV-1）病毒载量监测技术。本综述描述了目前在发展中国家评估和使用的三种替代HIV-1病毒载量方法的主要特点和优缺点，即超p24抗原检测、ExaVir Load逆转录酶活性检测和“自制”实时PCR HIV-1 RNA技术。本综述从与商用HIV-1 RNA检测的相关性、HIV-1基因多样性对定量的影响以及它们在儿科HIV-1感染早期诊断和高效抗逆转录病毒疗法疗效监测方面的有用性等方面，讨论了用这三种技术获得的临床结果。此外，还根据资源受限环境中的实验室设施，讨论了HIV-1病毒载量监测的不同策略。
