Qin Xiaoli, Ahn Soyeon, Speed Terence P, Rubin Gerald M
Howard Hughes Medical Institute, Department of Molecular & Cellular Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Genome Biol. 2007;8(4):R63. doi: 10.1186/gb-2007-8-4-r63.
In many animals, the first few hours of life proceed with little or no transcription, and developmental regulation at these early stages is dependent on maternal cytoplasm rather than the zygotic nucleus. Translational control is critical for early Drosophila embryogenesis and is exerted mainly at the gene level. To understand post-transcriptional regulation during Drosophila early embryonic development, we used sucrose polysomal gradient analyses and GeneChip analysis to illustrate the translation profile of individual mRNAs.
We determined ribosomal density and ribosomal occupancy of over 10,000 transcripts during the first ten hours after egg laying.
We report the extent and general nature of gene regulation at the translational level during early Drosophila embryogenesis on a genome-wide basis. The diversity of the translation profiles indicates multiple mechanisms modulating transcript-specific translation. Cluster analyses suggest that the genes involved in some biological processes are co-regulated at the translational level at certain developmental stages.
在许多动物中，生命最初的几个小时几乎没有转录，这些早期阶段的发育调控依赖于母体细胞质而非合子核。翻译控制对果蝇早期胚胎发育至关重要，且主要在基因水平发挥作用。为了解果蝇早期胚胎发育过程中的转录后调控，我们使用蔗糖多聚核糖体梯度分析和基因芯片分析来阐明单个mRNA的翻译概况。
我们确定了产卵后最初十小时内超过10000个转录本的核糖体密度和核糖体占有率。
我们在全基因组范围内报告了果蝇早期胚胎发育过程中翻译水平上基因调控的程度和一般性质。翻译概况的多样性表明存在多种调节转录本特异性翻译的机制。聚类分析表明，参与某些生物学过程的基因在特定发育阶段在翻译水平上受到共同调控。
