[肥大细胞在过敏性紫癜性肾炎患儿肾间质纤维化发生中的作用]

[Role of mast cells in the development of renal interstitial fibrosis in children with Henoch-Schonlein purpura nephritis].

作者信息

Zhang Gao-Ping, Dang Xi-Qiang, Yi Zhu-Wen, He Xiao-Jie, Zhang Jian-Jiang, Wu Xiao-Chuan, Mo Shuang-Hong

机构信息

Department of Respiratory, Children's Hospital of Jiangxi Province, Nanchang, China.

出版信息

Zhongguo Dang Dai Er Ke Za Zhi. 2007 Apr;9(2):125-8.

PMID:17448307

Abstract

OBJECTIVE

To investigate the role of mast cells in the development of renal interstitial fibrosis in children with Henoch-Schonlein purpura nephritis (HSPN) and possible mechanisms.

METHODS

Paraffin-embedded renal biopsy tissue sections from 20 children with HSPN were examined for the levels of tryptase-beta and transforming growth factor-beta1 (TGF-beta1) by immunohistochemical staining. Mast cells were counted by toluidine blue staining. Masson staining was used to assess the level of renal interstitial fibrosis and renal histopathological scores. Normal renal tissue sections from 5 nephrectomized children for nephroma were used as control group.

RESULTS

The percentages of positive tryptase-beta cellsand mast cells and the TGF-beta1 expression in the HSPN group were significantly higher than those in the control group (P < 0.05). The percentages of positive tryptase-beta cells and mast cells and the TGF-beta1 expression in renal tissue were positively correlated with the glomeruli histopathological score (r =0.940, 0.920, 0.937, respectively; P < 0.05) and were also positively correlated with the histopathological score of renal interstitium (r=0.903, 0.859, 0.948, respectively; P < 0.05). The level of renal interstitial fibrosis was positively correlated with the percentages of positive tryptase-beta cells and mast cells and the expression of TGF-beta1 (r =0.790, 0.766, 0.858, respectively; P < 0.05). There was a positive correlation between the percentages of positive tryptase-beta cells and mast cells (r =0.941, P < 0.05), between the percentage of positive tryptase-beta cells and the TGF-beta1 expression (r =0.897, P < 0.05) and between the percentage of positive mast cells and the TGF-beta1 expression (r=0.942, P < 0.05).

CONCLUSIONS

Tubulointerstitial mast cell infiltration is associated with the development of renal interstitial fibrosis in children with HSPN. Mast cells together with TGF-beta1 and mast cell-derived tryptase-beta may be involved in the development of the renal interstitial fibrosis in HSPN.

摘要

目的

探讨肥大细胞在过敏性紫癜性肾炎（HSPN）患儿肾间质纤维化发生发展中的作用及可能机制。

方法

采用免疫组织化学染色法检测20例HSPN患儿石蜡包埋肾活检组织切片中β-组织蛋白酶和转化生长因子-β1（TGF-β1）的水平。用甲苯胺蓝染色计数肥大细胞。采用Masson染色评估肾间质纤维化程度及肾脏组织病理学评分。选取5例因肾母细胞瘤行肾切除术患儿的正常肾组织切片作为对照组。

结果

HSPN组β-组织蛋白酶阳性细胞百分比、肥大细胞百分比及TGF-β1表达均显著高于对照组（P<0.05）。肾组织中β-组织蛋白酶阳性细胞百分比、肥大细胞百分比及TGF-β1表达与肾小球组织病理学评分呈正相关（r分别为0.940、0.920、0.937；P<0.05），与肾间质组织病理学评分也呈正相关（r分别为0.903、0.859、0.948；P<0.05）。肾间质纤维化程度与β-组织蛋白酶阳性细胞百分比、肥大细胞百分比及TGF-β1表达呈正相关（r分别为0.790、0.766、0.858；P<0.05）。β-组织蛋白酶阳性细胞百分比与肥大细胞百分比之间呈正相关（r=0.941，P<0.05），β-组织蛋白酶阳性细胞百分比与TGF-β1表达之间呈正相关（r=0.897，P<0.05），肥大细胞阳性百分比与TGF-β1表达之间呈正相关（r=0.942，P<0.05）。