Zhuang Shufei, Kelo Lisha, Nardi James B, Kanost Michael R
Department of Biochemistry, Kansas State University, Manhattan, KS 66506, USA.
Dev Comp Immunol. 2007;31(11):1159-67. doi: 10.1016/j.dci.2007.03.002. Epub 2007 Apr 2.
Neuroglian, a member of the L1 family of cell adhesion molecules (L1-CAMs), is expressed on surfaces of granular cells and a subset of large plasmatocytes of Manduca sexta that act as foci for hemocyte aggregation during the innate immune response. Neuroglian expressed on surfaces of transfected Sf9 cells induced their homophilic aggregation, with the aggregation being abolished in the presence of recombinant immunoglobulin (Ig) domains of neuroglian. Neuroglian and its Ig domains also can interact with hemocyte-specific integrin (HS integrin) as demonstrated with an enzyme-linked immunoassay and a surface plasmon resonance (SPR) assay. Neuroglian double-stranded (ds) RNA not only depresses expression of neuroglian in hemocytes but also depresses the cell-mediated encapsulation response of these hemocytes to foreign surfaces. After injection of a monoclonal antibody (MAb 3B11) into M. sexta larvae that recognizes the Ig domains of neuroglian, the cell-mediated encapsulation response of hemocytes was likewise inhibited. The Ig domains of neuroglian are involved in both homophilic and heterophilic interactions, and subsets of these six different Ig domains may affect different functions of neuroglian.
神经胶质蛋白是细胞黏附分子L1家族（L1-CAMs）的成员之一，在烟草天蛾颗粒细胞和一部分大型浆细胞的表面表达，这些细胞在先天免疫反应中作为血细胞聚集的焦点。转染的Sf9细胞表面表达的神经胶质蛋白可诱导其嗜同性聚集，而在存在神经胶质蛋白的重组免疫球蛋白（Ig）结构域时，这种聚集会被消除。通过酶联免疫测定和表面等离子体共振（SPR）测定表明，神经胶质蛋白及其Ig结构域也可与血细胞特异性整合素（HS整合素）相互作用。神经胶质蛋白双链（ds）RNA不仅会抑制血细胞中神经胶质蛋白的表达，还会抑制这些血细胞对外来表面的细胞介导的包囊反应。向烟草天蛾幼虫注射识别神经胶质蛋白Ig结构域的单克隆抗体（MAb 3B11）后，血细胞的细胞介导的包囊反应同样受到抑制。神经胶质蛋白的Ig结构域参与嗜同性和异嗜性相互作用，这六个不同Ig结构域的子集可能会影响神经胶质蛋白的不同功能。
