[利用自旋标记研究人血清白蛋白与青霉素的相互作用]

[Study of the interaction of human serum albumin with penicillins by means of spin labels].

作者信息

Kharakhonycheva N V, Rudzit E A, Raĭkhman L M, Moshkovskiĭ Iu Sh

出版信息

Antibiotiki. 1975 Oct;20(10):917-21.

Abstract

Interaction of 8 penicillin preparations with human serum albumin was studied with the spin-labels method and a probe. Correlation between the binding level of penicillins with human serum albumin and their effect on the spectrum of EPR of the spin-label attached to albumin was observed only with the use of a hydrophobic probe (radical III). The covalent attached marks and the hydrophobic probe may be used for rapid orienting estimation of pencillin interaction with albumin.

摘要

采用自旋标记法和一种探针研究了8种青霉素制剂与人血清白蛋白的相互作用。仅在使用疏水探针（自由基III）时，观察到青霉素与人血清白蛋白的结合水平与其对附着于白蛋白的自旋标记物的电子顺磁共振谱的影响之间存在相关性。共价连接标记物和疏水探针可用于快速定向评估青霉素与白蛋白的相互作用。