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反刍月形单胞菌中乳酸脱氢酶特异性和生长速率与乳酸代谢的关系

Relationship of lactate dehydrogenase specificity and growth rate to lactate metabolism by Selenomonas ruminantium.

出版信息

Appl Microbiol. 1975 Dec;30(6):916-21. doi: 10.1128/am.30.6.916-921.1975.

Abstract

A lactate-fermenting strain of Selenomonas ruminantium (HD4) and a lactatenonfermenting strain (GA192) were examined with respect to the stereoisomers of lactate formed during glucose fermentation, the stereoisomers of lactate fermented by HD4, and the characteristics of the lactate dehydrogenases of the strains. GA192 formed L-lactate and HD4 formed L-lactate and small amounts of D-lactate from glucose. HD4 fermended L- but not D-lactate. Both strains contain nicotinamide adenine dinucleotide (NAD)-specific lactate dehydrogenases, and no NAD-independent lactate oxidation was detected. Continuous cultures of both strains grown with limiting glucose produced mainly propionate and acetate and little lactate at dilution rates less than 0.4/h, with shifts to increasing amounts of lactate and less acetate and propionate as the dilution rate was increased from 0.4/h to approximately 1/h.

摘要

研究了一株发酵乳酸的反刍月形单胞菌(HD4)和一株不发酵乳酸的菌株(GA192),内容涉及葡萄糖发酵过程中形成的乳酸的立体异构体、HD4发酵的乳酸立体异构体以及这些菌株的乳酸脱氢酶特性。GA192从葡萄糖中形成L-乳酸,HD4从葡萄糖中形成L-乳酸和少量D-乳酸。HD4发酵L-乳酸而非D-乳酸。两株菌均含有烟酰胺腺嘌呤二核苷酸(NAD)特异性乳酸脱氢酶,未检测到不依赖NAD的乳酸氧化。以限量葡萄糖培养的两株菌的连续培养物,在稀释率低于0.4/h时主要产生丙酸和乙酸,乳酸很少,随着稀释率从0.4/h增加到约1/h,乳酸量增加,乙酸和丙酸量减少。

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