储存温度对宫颈黏液中细胞因子稳定性的影响。
Effect of storage temperatures on the stability of cytokines in cervical mucous.
作者信息
Panicker Gitika, Meadows Kristi S, Lee Daisy R, Nisenbaum Rosane, Unger Elizabeth R
机构信息
Centers for Disease Control and Prevention, Division of Viral and Rickettsial Diseases, Viral Exanthems and Herpesvirus Branch, Atlanta, GA 30333, USA.
出版信息
Cytokine. 2007 Feb;37(2):176-9. doi: 10.1016/j.cyto.2007.03.006. Epub 2007 Apr 20.
Abstract
Cytokines have progressively come to serve as indicators for the presence or severity of a disease. But accurate measurement of cytokine levels can be deterred by lack of proper handling and storage of the samples. In this study, we attempted to measure the effect of snap-freezing and refrigeration at the time of collection on cervical mucous. Luminex analyses of the frozen and refrigerated pairs exhibited no significant differences in levels for 7 out of the 10 cytokines measured simultaneously. The cytokines TNF-alpha, IFN-gamma and IL-1beta, were significantly different between the pairs with the refrigerated samples showing higher levels for each of these cytokines. The results suggest that refrigeration of mucous samples immediately after collection would allow for better conservation of the cytokines in cervical mucous.
摘要
细胞因子已逐渐成为疾病存在或严重程度的指标。但由于样本处理和储存不当，可能会妨碍细胞因子水平的准确测量。在本研究中，我们试图测量采集时速冻和冷藏对宫颈黏液的影响。对冷冻和冷藏样本进行的Luminex分析显示，同时检测的10种细胞因子中有7种在水平上无显著差异。细胞因子TNF-α、IFN-γ和IL-1β在两组样本之间存在显著差异，冷藏样本中这些细胞因子的水平更高。结果表明，采集后立即冷藏黏液样本有助于更好地保存宫颈黏液中的细胞因子。
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