Jo Won-Min, Sohn Young-sang, Choi Young Ho, Kim Hark Jei, Cho Hyun Deuk
Department of Thoracic and Cardiovascular Surgery, Ansan Hospital, Korea University, Ansan, Korea.
J Korean Med Sci. 2007 Apr;22(2):262-9. doi: 10.3346/jkms.2007.22.2.262.
The purpose of this study was to estimate the possibilities of an acellular matrix using a modified acellularization protocol, which circumvents immunological, microbiological, and physiological barriers. We treated porcine subclavian arteries with various reagents to construct acellular grafts. Afterwards, these grafts were interposed in a mongrel dogs' abdominal aorta. Six dogs underwent interposition with fresh porcine grafts (control group), and seven had interposed acellular grafts (acellular group). The control and acellular group dogs were sacrificed at 1, 3, 5 (n=2 in each group) and 12 months (n=1 in acellular group) after the operation. Histopathological examinations were then performed, to assess the degree to which re-endothelialization, inflammation, thrombus formation, and calcification occurred. The entire acellular group, but none of the control group, exhibited re-endothelialization. The degrees to which inflammation, thrombosis, and calcification occurred were found to be lower in the acellular group. We also discovered many smooth muscle cells in the medial layer of the xenograft that had been implanted in the dog sacrificed 12 months after the operation. These results suggest that the construction of xenografts using our modified acellularization protocol may offer acceptable outcomes as a vascular xenograft.
本研究的目的是评估采用改良脱细胞方案制备脱细胞基质的可能性，该方案可规避免疫、微生物和生理屏障。我们用各种试剂处理猪锁骨下动脉以构建脱细胞移植物。之后，将这些移植物植入杂种犬的腹主动脉。6只犬植入新鲜猪移植物（对照组），7只犬植入脱细胞移植物（脱细胞组）。对照组和脱细胞组的犬在术后1、3、5个月（每组n = 2）和12个月（脱细胞组n = 1）时处死。然后进行组织病理学检查，以评估再内皮化、炎症、血栓形成和钙化发生的程度。整个脱细胞组均出现了再内皮化，而对照组均未出现。发现脱细胞组炎症、血栓形成和钙化的程度较低。我们还在术后12个月处死的犬体内植入的异种移植物中层发现了许多平滑肌细胞。这些结果表明，使用我们改良的脱细胞方案构建异种移植物作为血管异种移植物可能会有可接受的结果。
