Lee Hak-Mo, Oh Byoung Chol, Lim Dong-Pyo, Lee Dong-Sup, Cho Jaejin, Lee Gene, Lee Jeong Ryul
Department of Thoracic and Cardiovascular Surgery, College of Medicine, Seoul National University, Seoul, Korea.
J Korean Med Sci. 2007 Apr;22(2):277-82. doi: 10.3346/jkms.2007.22.2.277.
Sertoli cells (SC) are known to contain immunoprotective properties, which allow them to survive as allografts without the use of immunosuppressive drugs. Experiments were designed to determine which factors are related to prolonged survival of allogeneic SC. Balb/c derived Sertoli (TM4) and colon cancer (CT-26) cell lines were implanted beneath the kidney capsule of non-immunosuppressed C57BL/6 mice and compared their survival as allografts. Compared to TM4 graft, which survived more than 7 days after transplantation, CT-26 showed massive infiltration of polymorphonuclear cells, necrosis and enlargement of draining lymph nodes. Cultured cell lines showed no differences in their expression patterns of FasL, TGF beta1, clusterin and two complement regulatory proteins (CRP, i.e., membrane cofactor protein, MCP; decay accelerating factor, DAF), but protectin (CD59), another member of CRP was expressed only on TM4. These results suggest that CD59 and unknown factors may contribute to the prolonged survival of SC in non-immunoprivileged sites.
已知支持细胞（SC）具有免疫保护特性，这使得它们作为同种异体移植物能够在不使用免疫抑制药物的情况下存活。实验旨在确定哪些因素与同种异体支持细胞的长期存活有关。将源自Balb/c的支持细胞（TM4）和结肠癌细胞系（CT-26）植入未免疫抑制的C57BL/6小鼠的肾包膜下，并比较它们作为同种异体移植物的存活情况。与移植后存活超过7天的TM4移植物相比，CT-26显示多形核细胞大量浸润、坏死以及引流淋巴结肿大。培养的细胞系在FasL、转化生长因子β1、簇集素和两种补体调节蛋白（CRP，即膜辅因子蛋白，MCP；衰变加速因子，DAF）的表达模式上没有差异，但CRP的另一个成员保护素（CD59）仅在TM4上表达。这些结果表明，CD59和未知因素可能有助于支持细胞在非免疫赦免部位的长期存活。
