Sasaki Yasnory T F, Sano Miho, Ideue Takashi, Kin Taishin, Asai Kiyoshi, Hirose Tetsuro
Functional RNA Research Team, Biological Information Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tokyo 135-0064, Japan.
Biochem Biophys Res Commun. 2007 Jun 15;357(4):991-6. doi: 10.1016/j.bbrc.2007.04.034. Epub 2007 Apr 17.
We have examined the expression profile of selected non-coding RNAs (ncRNAs) in 11 human tissues. Among 5489 full-length cDNA clones annotated as non-protein-coding transcripts in the H-Invitational database, we chose 150 clones for further analysis based on their gene structure and EST information. Expression profiling using quantitative RT-PCR and Northern blot hybridization revealed that the majority of the selected ncRNAs exhibited tissue specificity: 67% are predominantly expressed in a restricted subset of tissues. The absolute quantification of representative ncRNAs revealed that the majority of ncRNAs are expressed as low abundance transcripts. A comparative genomic analysis revealed that only 27% of the selected ncRNAs have mouse counterparts. Since the expression patterns of the human ncRNAs having no mouse counterparts remain to be similar to those of the mouse ncRNAs, the expression patterns of the selected ncRNAs may be conserved between human and mouse.
我们检测了11种人体组织中选定的非编码RNA（ncRNA）的表达谱。在H-Invitational数据库中注释为非蛋白质编码转录本的5489个全长cDNA克隆中，我们根据其基因结构和EST信息选择了150个克隆进行进一步分析。使用定量RT-PCR和Northern印迹杂交进行的表达谱分析表明，大多数选定的ncRNA表现出组织特异性：67%主要在有限的组织亚组中表达。代表性ncRNA的绝对定量显示，大多数ncRNA以低丰度转录本形式表达。比较基因组分析表明，选定的ncRNA中只有27%有小鼠对应物。由于没有小鼠对应物的人类ncRNA的表达模式仍与小鼠ncRNA的表达模式相似，因此选定的ncRNA的表达模式可能在人和小鼠之间保守。
