Wang Meichi, Guan Xin, Liang Songping
Key Laboratory of Protein Chemistry and Developmental Biology of Education, The College of Life Science, Hunan Normal University, Changsha 410081, China.
Biochem Biophys Res Commun. 2007 Jun 8;357(3):579-83. doi: 10.1016/j.bbrc.2007.02.168. Epub 2007 Apr 9.
In this paper, we investigated the action of huwentoxin-I (HWTX-I) purified from the venom of the Chinese bird spider Ornithoctonus huwena on Ca(2+), Na(+) channels of adult rat dorsal root ganglion (DRG) neurons. The results showed that huwentoxin-I could reduce the peak currents of N-type Ca(2+) channels (IC(50) approximately 100 nM) and TTX-S Na(+) channels (IC(50) approximately 55 nM), whereas no effect was detected on TTX-R Na(+) channels. The comparative studies indicated that the selectivity of HWTX-I on Ca(2+) channels was higher that of MVIIA and approximately the same as that of GVIA. HWTX-I is the first discovered toxin with the cross channel activities from the spider O. huwena venom similar to micro O-conotoxins MrVIA and MrVIB.
在本文中，我们研究了从中国鸟蛛虎纹捕鸟蛛毒液中纯化得到的虎纹毒素-I（HWTX-I）对成年大鼠背根神经节（DRG）神经元钙通道和钠通道的作用。结果表明，虎纹毒素-I可降低N型钙通道（IC50约为100 nM）和TTX-S钠通道（IC50约为55 nM）的峰值电流，而对TTX-R钠通道无影响。比较研究表明，HWTX-I对钙通道的选择性高于MVIIA，与GVIA大致相同。HWTX-I是首次从虎纹捕鸟蛛毒液中发现的具有类似微小O-芋螺毒素MrVIA和MrVIB跨通道活性的毒素。
