Paszkoand Z, Padzik H
Arch Geschwulstforsch. 1975;45(5):430-43.
The specific estrogen binding capacity of the cytosol has been investigated in 51 human breast cancers (49 women, 2 men). The estrogen binding sites of the cytosol has been analysed by Korenman's dextrancoated charcoal technique. Several methods have been used in order to distinguish the specific estrogen binding sites and the unspecific binding: temperature differentiation and saturation of the binding sites with nonlabelled estrogen or its antagonist Clomid. It has been concluded that the differentiation of the estradiol binding sites (specific or unspecific) with the elevated temperature effect or the additon of Clomid may be helpful only as a qualitative test. The elevated temperature of Clomid may influence the increase of the unspecific estradiol binding. The most suitable method to eliminate the unspecific binding of estradiol is the saturation of cytosol from control samples with non-radioactive estradiol-17-beta. The specific estrogen binding capacity of the cytosols of different tumours varied over a wide range (0--840 fM E2/mg protein). It has been found that among 51 persons there were 31 patients (61%) with measureable amounts of high affinity estradiol binding sites in cancerous tissues. In 20 cases (39%) the existence of estradiol specific binding sites could not be observed.
对51例人类乳腺癌（49名女性，2名男性）的胞质溶胶的特异性雌激素结合能力进行了研究。采用科伦曼的葡聚糖包被活性炭技术分析了胞质溶胶的雌激素结合位点。为了区分特异性雌激素结合位点和非特异性结合，使用了几种方法：温度差异以及用未标记的雌激素或其拮抗剂克罗米酚使结合位点饱和。得出的结论是，利用温度升高效应或添加克罗米酚来区分雌二醇结合位点（特异性或非特异性）仅作为一种定性测试可能有用。温度升高或克罗米酚可能会影响非特异性雌二醇结合的增加。消除雌二醇非特异性结合的最合适方法是用非放射性雌二醇 - 17 - β使对照样品的胞质溶胶饱和。不同肿瘤胞质溶胶的特异性雌激素结合能力在很宽的范围内变化（0 - 840 fM E2/毫克蛋白质）。已发现，在51人中，有31例患者（61%）癌组织中存在可测量量的高亲和力雌二醇结合位点。在20例（39%）中未观察到雌二醇特异性结合位点的存在。
