利用多孔石墨化碳通过液相色谱-大气压化学电离质谱法分析硝酸酯、硝胺和硝基芳香族炸药及其副产物。

Use of porous graphitic carbon for the analysis of nitrate ester, nitramine and nitroaromatic explosives and by-products by liquid chromatography-atmospheric pressure chemical ionisation-mass spectrometry.

作者信息

Tachon Romain, Pichon Valérie, Le Borgne Martine Barbe, Minet Jean-Jacques

机构信息

Laboratoire Central de la Préfecture de Police, 39 bis rue de Dantzig, 75015 Paris, France.

出版信息

J Chromatogr A. 2007 Jun 22;1154(1-2):174-81. doi: 10.1016/j.chroma.2007.03.059. Epub 2007 Mar 21.

DOI:10.1016/j.chroma.2007.03.059

PMID:17451723

Abstract

A new LC/MS method was developed for the analysis of sixteen different analytes including the most common organic explosives encountered in forensic investigations. The separation was achieved using a porous graphitic carbon (PGC) column with a binary gradient elution. Molecular modeling suggested a possible interpretation for the elution order of explosive compounds on PGC. The introduction of ammonium formate in the mobile phase resulted in the formation of characteristic adduct ions thus enhancing the mass spectrometric detection of nitrate ester and nitramine compounds. Atmospheric pressure chemical ionization (APCI) and electrospray ionization (ESI) were compared in terms of sensitivity. The final LC/APCI-MS method allowed easy identification of investigated compounds with limits of detection ranging from 0.04 to 1.06 ng/microl. The analysis of simulated forensic samples confirmed the performance of the method.

摘要

开发了一种新的液相色谱/质谱（LC/MS）方法，用于分析16种不同的分析物，包括法医调查中最常见的有机炸药。使用带有二元梯度洗脱的多孔石墨化碳（PGC）柱实现分离。分子模拟为PGC上炸药化合物的洗脱顺序提供了一种可能的解释。在流动相中引入甲酸铵导致形成特征性加合离子，从而增强了硝酸酯和硝胺化合物的质谱检测。比较了大气压化学电离（APCI）和电喷雾电离（ESI）的灵敏度。最终的LC/APCI-MS方法能够轻松鉴定所研究的化合物，检测限范围为0.04至1.06纳克/微升。对模拟法医样本的分析证实了该方法的性能。