Ventriglia Tiziana, Ballicora Miguel A, Crevillén Pedro, Preiss Jack, Romero José M
Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, Universidad de Sevilla-CSIC, c/Americo Vespucio No. 49, 41092-Sevilla, Spain.
Plant Cell Physiol. 2007 Jun;48(6):875-80. doi: 10.1093/pcp/pcm047. Epub 2007 Apr 22.
In higher plants, ADP-glucose pyrophosphorylase (ADPGlc-PPase) is a heterotetrameric enzyme comprised of two small and two large subunits. Potato-Arabidopsis hybrid ADPGlc-PPases were generated and their regulatory properties analyzed. We show that ADPGlc-PPase subunits from two different species can interact, producing active enzymes with new regulatory properties. Depending on the subunit combinations, hybrid heterotetramers showed responses to allosteric effectors [3-phosphoglycerate (3-PGA) and Pi] in the micromolar or millimolar range. While hybrid potato small subunit (PSS) and the Arabidopsis large subunit APL1 showed an extremely sensitive response to 3-PGA and Pi, hybrid PSS/Arabidopsis APL2 was very insensitive to them. Intermediate responses were determined for other subunit combinations.
在高等植物中，ADP - 葡萄糖焦磷酸化酶（ADPGlc - PPase）是一种由两个小亚基和两个大亚基组成的异源四聚体酶。构建了马铃薯 - 拟南芥杂交ADPGlc - PPases并分析了它们的调控特性。我们发现来自两个不同物种的ADPGlc - PPase亚基能够相互作用，产生具有新调控特性的活性酶。根据亚基组合的不同，杂交异源四聚体对微摩尔或毫摩尔浓度范围的变构效应物[3 - 磷酸甘油酸（3 - PGA）和无机磷酸（Pi）]表现出不同的响应。杂交马铃薯小亚基（PSS）和拟南芥大亚基APL1对3 - PGA和Pi表现出极其敏感的响应，而杂交PSS/拟南芥APL2对它们则非常不敏感。其他亚基组合表现出中间响应。
