Huang Zuo-Xi, Duan Hui-Guo, Qing Dong-Hong, Wang Fang, Liu Lan
Department of Chemistry and Life Science, Flower Research Institute, Neijiang Teachers College, Neijiang, Sichuan 641112, China.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2007 Apr;33(2):160-4.
This paper reported the synergistic effect of KT and IAA, and the effects of age of seedlings, length of hypocotyls, nitrogen contents and NH(+)(4)-N/TN ratio on the induction of female flowers in derooted seedlings of Cucumis sativus L. for the establishment of an efficient system for the induction of female flowers. When cultured on MS basal medium supplemented with KT 3.0 mg/L and IAA 0.01 mg/L, an induction rate of 28% was obtained for the 7-day-old derooted seedlings with 1/2 hypocotyl, 12% and 26% higher than when cultured with KT 3.0 mg/L alone and IAA 0.01 mg/L alone, respectively, while no female flowers was formed on the control medium, which indicates that KT and IAA have an evident synergistic effect on the induction of female flowers. Experiment was done with N concentration of 60-90 mmol/L or with NH(+)(4)-N/TN ratio of 12.5%-50% (total N kept at 80 mmol/L), with lengths of hypocotyls of 0-4/4, and with different ages of seedlings of 5-8-day-old, the highest induction rate of 40%, 48% and 57% for female flowers was achieved on medium of N 80 mmol/L (NH(+)(4)-N/TN 37.5%), from seedlings with 1/4 hypocotyl and from the treatment using 6-day-old seedlings respectively.
本文报道了激动素（KT）和吲哚乙酸（IAA）的协同效应，以及黄瓜去根苗中苗龄、下胚轴长度、氮含量和铵态氮/总氮比值对雌花诱导的影响，旨在建立高效的雌花诱导体系。在添加3.0 mg/L KT和0.01 mg/L IAA的MS基本培养基上培养时，7日龄、下胚轴长度为1/2的去根苗雌花诱导率为28%，分别比单独添加3.0 mg/L KT和单独添加0.01 mg/L IAA时高出12%和26%，而对照培养基上未形成雌花，这表明KT和IAA对雌花诱导具有明显的协同效应。实验设置了氮浓度为60 - 90 mmol/L或铵态氮/总氮比值为12.5% - 50%（总氮保持在80 mmol/L）、下胚轴长度为0 - 4/4以及苗龄为5 - 8日龄等条件，分别在氮浓度为80 mmol/L（铵态氮/总氮37.5%）的培养基上、下胚轴长度为1/4的苗以及使用6日龄苗的处理中，雌花诱导率最高分别达到40%、48%和57%。
