[来自耐盐胡杨（Populus euphratica Oliv）的假定Na⁺/H⁺逆向转运蛋白基因PeNhaD1的功能]
[Function of the putative Na+/H+ antiporter gene PeNhaD1 from salt-resistant Populus euphratica Oliv].
作者信息
Lv Ping-Ping, Hu Jun, Chen Shao-Liang, Shen Xin, Yin Wei-Bo, Chen Yu-Hong, Sun Yong-Ru, Hu Zan-Min
机构信息
College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
出版信息
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2007 Apr;33(2):173-8.
Yeast complementation experiments were carried out to define the possible function of PeNhaD1, a Na(+)/H(+) antiporter gene from Populus euphratica Oliv., a salt resistant tree. PeNhaD1 was introduced to the Saccharomyces cerevisiae mutant strain ANT3 (Deltaena1-4::HIS3 Deltanha1::LEU2), which lacks the plasma membrane Na(+)/H(+) antiporter gene ScNHA1 or GX1 (Deltanhx1::TRP1), which lacks tonoplast Na(+)/H(+) antiporter gene ScNHX1. Our results showed that PeNhaD1 rescued the normal growth of ANT3 in the presence of high salt (80 mmol/L NaCl on solid medium or 400 mmol/L in liquid medium, pH 6.0), but not that of GX1, suggesting that PeNhaD1 may play a role in salt tolerance of Populus euphratica by maintaining the capacity for salt exclusion under saline condition.
进行了酵母互补实验，以确定胡杨（一种耐盐树木）的Na(+)/H(+)逆向转运蛋白基因PeNhaD1的可能功能。将PeNhaD1导入酿酒酵母突变株ANT3（Deltaena1-4::HIS3 Deltanha1::LEU2），该菌株缺乏质膜Na(+)/H(+)逆向转运蛋白基因ScNHA1，或导入GX1（Deltanhx1::TRP1），该菌株缺乏液泡膜Na(+)/H(+)逆向转运蛋白基因ScNHX1。我们的结果表明，在高盐条件下（固体培养基中为80 mmol/L NaCl，液体培养基中为400 mmol/L，pH 6.0），PeNhaD1挽救了ANT3的正常生长，但不能挽救GX1的正常生长，这表明PeNhaD1可能通过在盐胁迫条件下维持排盐能力，在胡杨耐盐性中发挥作用。
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