利用灌注大鼠肝脏研究极低密度脂蛋白的三酰甘油与蛋白质成分之间的相互关系。
A study of the interrelationship between the triacylglycerol and protein components of very-low-density lipoproteins using the perfused rat liver.
作者信息
Petersburg S J, Madeley A, Robinson D S
出版信息
Biochem J. 1975 Sep;150(3):315-21. doi: 10.1042/bj1500315.
High and low rates of very-low-density-lipoprotein triacylglycerol release from the perfused rat liver were achieved by using livers taken respectively from animals that had been given fructose for 48h or from animals that had been starved for 18h. 2. The higher rates of very-low-density-lipoprotein triacylglycerol release by the livers of the fructose-fed rats were associated with higher rates of very-low-density-lipoprotein protein release. 3. When the livers were perfused in the presence of [3H]leucine, radioactivity was incorporated into the very-low-density-lipoprotein apoproteins. The higher rates of very-low-density-lipoprotein triacylgycerol and protein release by the livers of fructose-fed rats were associated with a greater total incorporation of radioactivity into those apoproteins that entered the running gel during polyacrylamide-gel electrophoresis. However, the distribution of radioactivity among the various apoproteins was not significantly changed by the dietary treatments used.
通过分别使用来自经48小时果糖喂养的动物或经18小时饥饿的动物的肝脏，实现了灌注大鼠肝脏极低密度脂蛋白三酰甘油释放的高、低速率。2. 果糖喂养大鼠的肝脏释放极低密度脂蛋白三酰甘油的较高速率与极低密度脂蛋白蛋白质释放的较高速率相关。3. 当肝脏在[3H]亮氨酸存在下进行灌注时，放射性被掺入极低密度脂蛋白载脂蛋白中。果糖喂养大鼠的肝脏释放极低密度脂蛋白三酰甘油和蛋白质的较高速率与在聚丙烯酰胺凝胶电泳期间进入运行凝胶的那些载脂蛋白中放射性的更大总掺入量相关。然而，饮食处理并未显著改变放射性在各种载脂蛋白之间的分布。
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