Jørgensen J M, Sørensen F B, Bendix K, Nielsen J L, Olsen M L, Funder A M D, d'Amore F
Department of Haematology, Aarhus University Hospital, Arhus, Denmark.
Leuk Lymphoma. 2007 Mar;48(3):584-95. doi: 10.1080/10428190601083241.
The aim of the study was to evaluate angiogenesis in different subtypes of non-Hodgkin's lymphoma (NHL) and to correlate angiogenic scores to clinical endpoints. Pre-therapeutic lymph node biopsies from 308 patients with NHL [107 follicular B-cell lymphoma (FL), 94 diffuse large B-cell lymphoma (DLBCL), 107 peripheral T-cell lymphoma (PTCL)] were studied. Microvessels were scored according to the Chalkley and microvessel density method (MVD) methods. Vascular endothelial growth factor (VEGF) protein expression was evaluated by immunohistochemistry. Both Chalkley and MVD methods showed, that the lymphoma subtypes differed significantly in angiogenic scores (P < 0.001). Angiogenic scores in tumor area were highest in PTCL, and lowest in FL. However, a remarkable high microvessel density was found in interfollicular areas of FL. In FL, high interfollicular MVD scores predicted progressive disease and poorer overall and event-free survival (P = 0.024 and 0.013). High interfollicular Chalkley scores correlated with transformation to DLBCL (P = 0.01). VEGF expression was detected in all NHL subtype, and the strongest expression was found in PTCL. In FL, patients with diffuse VEGF expression in lymphoma cells had poorer overall survival than those with focal expression.
本研究的目的是评估不同亚型非霍奇金淋巴瘤（NHL）中的血管生成情况，并将血管生成评分与临床终点相关联。对308例NHL患者[107例滤泡性B细胞淋巴瘤（FL）、94例弥漫性大B细胞淋巴瘤（DLBCL）、107例外周T细胞淋巴瘤（PTCL）]的治疗前淋巴结活检标本进行了研究。微血管根据Chalkley法和微血管密度法（MVD）进行评分。通过免疫组织化学评估血管内皮生长因子（VEGF）蛋白表达。Chalkley法和MVD法均显示，淋巴瘤亚型的血管生成评分存在显著差异（P<0.001）。肿瘤区域的血管生成评分在PTCL中最高，在FL中最低。然而，在FL的滤泡间区域发现了显著高的微血管密度。在FL中，滤泡间高MVD评分预示疾病进展以及较差的总生存期和无事件生存期（P=0.024和0.013）。滤泡间高Chalkley评分与向DLBCL转化相关（P=0.01）。在所有NHL亚型中均检测到VEGF表达，其中PTCL中的表达最强。在FL中，淋巴瘤细胞中VEGF弥漫性表达的患者总生存期比局灶性表达的患者差。
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