Shahini Labinot, Gašparov Slavko, Petruševska Gordana, Manxhuka Kerliu Suzana, Veselaj Fahredin, Kurshumliu Fisnik, Kavaja Floren
Institute of Pathology, Faculty of Medicine, University of Prishtina, Prishtina, Kosovo.
Department of Pathology and Cytology, Merkur University Hospital, School of Medicine, University of Zagreb, Zagreb, Croatia.
Acta Clin Croat. 2017 Dec;56(4):588-593. doi: 10.20471/acc.2017.56.04.02.
Angiogenesis is essential for the development, growth and progression of tumors. Although vascular endothelial growth factor (VEGF) is a well-known proangiogenic factor, its impact on lymphoma has not yet been fully clarified. The aim of this study was to evaluate VEGF-A -expression and microvessel density (MVD) in aggressive lymphoma such as diffuse large B-cell lymphoma (DLBCL), in indolent lymphomas such as low-grade follicular lymphoma (FL), and in lymph node reactive follicular hyperplasia (FH). In 80 prospective and retrospective cases (30 DLBCL, 30 FL and 20 FH), CD31 was analyzed by immunohistochemical staining assessing density of blood vessels, as well as the total number of CD31 positive endothelial cells. The results were compared with relevant clinical data. MVD was 85% in FH, followed by 60% in DLBCL and 43% in low-grade FL. VEGF-A was significantly higher in DLBCL than in low-grade FL and FH. A statistically significant association of MVD and VEGF-A with the International Prognostic Index (IPI) was found in DLBCL. High MVD and VEGF-A expression was observed in DLBCL patients with high IPI, while there was no statistically significant association between MVD and VEGF-A with the Follicular Lymphoma International Prognostic Index in low-grade FL. Our results suggested an important relationship between angiogenesis and high-grade lymphoma.
血管生成对于肿瘤的发生、生长和进展至关重要。虽然血管内皮生长因子（VEGF）是一种众所周知的促血管生成因子，但其对淋巴瘤的影响尚未完全阐明。本研究的目的是评估VEGF-A在侵袭性淋巴瘤（如弥漫性大B细胞淋巴瘤（DLBCL））、惰性淋巴瘤（如低级别滤泡性淋巴瘤（FL））以及淋巴结反应性滤泡增生（FH）中的表达和微血管密度（MVD）。在80例前瞻性和回顾性病例（30例DLBCL、30例FL和20例FH）中，通过免疫组织化学染色分析CD31，评估血管密度以及CD31阳性内皮细胞的总数。将结果与相关临床数据进行比较。FH中的MVD为85%，其次是DLBCL中的60%和低级别FL中的43%。DLBCL中的VEGF-A显著高于低级别FL和FH。在DLBCL中发现MVD和VEGF-A与国际预后指数（IPI）存在统计学显著关联。高IPI的DLBCL患者中观察到高MVD和VEGF-A表达，而在低级别FL中，MVD和VEGF-A与滤泡性淋巴瘤国际预后指数之间无统计学显著关联。我们的结果表明血管生成与高级别淋巴瘤之间存在重要关系。
