Zhang Liang-feng, Tian Xiao-xiao, Wang Chun-feng
Digestive Diseases Department, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
Zhonghua Gan Zang Bing Za Zhi. 2007 Apr;15(4):258-60.
To detect the expressions of serum transforming growth factor beta (TGFbeta1) and tissue inhibitor of metalloproteinase 1 (TIMP-1) in patients with chronic hepatitis B (CHB) in different phases of their liver fibrosis and to investigate the effects of TGFbeta1 and TIMP-1 on the diagnosis of hepatic fibrosis and the relationship between them.
Serum TGFbeta1 and TIMP-1 were detected by enzyme-linked immunosorbent assay and serum HA, LN, PIIINP were detected by radioimmunoassay in 40 CHB patients and 12 healthy persons. Liver biopsies were performed on the 40 patients. The relationships between the TGFbeta1 and TIMP-1 and hepatic fibrosis stages, hepatitis grades and the relevance of the five serum makers of the patients were analyzed.
Serum TGFbeta1, TIMP-1 rose in the early stage of liver fibrosis and their sensitivities were higher than that of HA, LN and PIIINP. Serum levels of TGFbeta1 and TIMP-1 increased significantly with the development of fibrosis and had positive correlations with other fibrotic makers such as HA, LN and PIIINP.
TGFbeta1 and TIMP-1 are involved in the course of developing hepatic fibrosis and TIMP-1 may be regulated by TGFbeta1. Both of them can be used as diagnostic markers of early hepatic fibrosis.
检测慢性乙型肝炎（CHB）患者不同肝纤维化阶段血清转化生长因子β（TGFβ1）和基质金属蛋白酶组织抑制因子1（TIMP-1）的表达，探讨TGFβ1和TIMP-1对肝纤维化诊断的作用及其相互关系。
采用酶联免疫吸附法检测40例CHB患者及12例健康人血清TGFβ1和TIMP-1，采用放射免疫法检测血清透明质酸（HA）、层粘连蛋白（LN）、Ⅲ型前胶原肽（PIIINP）。对40例患者进行肝穿刺活检。分析TGFβ1和TIMP-1与肝纤维化分期、肝炎分级及患者血清五项指标的相关性。
血清TGFβ1、TIMP-1在肝纤维化早期升高，其敏感性高于HA、LN和PIIINP。血清TGFβ1和TIMP-1水平随纤维化进展显著升高，与HA、LN和PIIINP等其他纤维化指标呈正相关。
TGFβ1和TIMP-1参与肝纤维化的发生发展过程，TIMP-1可能受TGFβ1调控。二者均可作为早期肝纤维化的诊断标志物。
