Kapás L, Obál F, Farkas I, Payne L C, Sáry G, Rubicsek G, Krueger J M
Department of Physiology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Physiol Behav. 1991 Aug;50(2):417-20. doi: 10.1016/0031-9384(91)90088-6.
It has been reported that systemic injections of cholecystokinin (CCK) elicit the behavioral characteristics of satiety, including sleep, in rats. CCK is a potent stimulator of insulin secretion, and insulin is hypothesized to be involved in sleep and feeding regulation. The purpose of the current experiments was to study the possible role of endogenous insulin in the food-intake-reducing and hypnogenic effects of intraperitoneally (IP) administered CCK. Normal and streptozotocin (STR)-diabetic rats were injected with isotonic saline or CCK (10 and 50 micrograms/kg) at dark onset, and sleep-wake activity was determined for the next 12 h. There were no significant differences between the baseline sleep-wake activity of normal and diabetic rats. IP injection of CCK elicited a selective increase in nonrapid-eye-movement sleep in both groups during the first postinjection hour. In a separate experiment, the effects of CCK (10 micrograms/kg) on food intake were determined in control and diabetic rats; CCK suppressed the 1-h food intake in both groups. In a third experiment, the effects of CCK treatment (50 micrograms/kg) on plasma insulin levels were determined. In normal rats, CCK elicited a two-fold increase in plasma insulin concentration, whereas diabetic rats had a significantly lower basal insulin level which was not affected by CCK treatment. We conclude that hypnogenic and food-intake-reducing effects of exogenously administered CCK are closely associated; however, pancreatic insulin does not play a significant role in either of these effects.
据报道，给大鼠全身注射胆囊收缩素（CCK）会引发饱腹感的行为特征，包括睡眠。CCK是胰岛素分泌的有效刺激物，并且胰岛素被认为参与睡眠和进食调节。当前实验的目的是研究内源性胰岛素在腹腔注射（IP）CCK的减少食物摄入和促眠作用中可能发挥的作用。在黑暗开始时，给正常大鼠和链脲佐菌素（STR）诱导的糖尿病大鼠注射等渗盐水或CCK（10和50微克/千克），并在接下来的12小时内测定睡眠-觉醒活动。正常大鼠和糖尿病大鼠的基线睡眠-觉醒活动之间没有显著差异。腹腔注射CCK在注射后的第一个小时内使两组的非快速眼动睡眠选择性增加。在另一个实验中，测定了CCK（10微克/千克）对对照大鼠和糖尿病大鼠食物摄入量的影响；CCK抑制了两组的1小时食物摄入量。在第三个实验中，测定了CCK处理（50微克/千克）对血浆胰岛素水平的影响。在正常大鼠中，CCK使血浆胰岛素浓度增加了两倍，而糖尿病大鼠的基础胰岛素水平显著较低，且不受CCK处理的影响。我们得出结论，外源性给予的CCK的促眠和减少食物摄入的作用密切相关；然而，胰腺胰岛素在这两种作用中均未发挥重要作用。
