Park C E, Rayman M K, Stankiewicz Z K, Hauschild A H
Can J Microbiol. 1977 May;23(5):559-62. doi: 10.1139/m77-080.
Trypticase soy broth was superior to nutrient and lactose broths as a preenrichment medium for the detection of Salmonella in artificially and naturally contaiminated gelatin. The detection rate for Salmonella were further enhanced when homogenization of the gelatin-broth mixture was accomplished by the use of gelatinase rather than by heating of 45 degrees C. Detection rats were also increased by adjusting the pH of the gelatin-broth mixture of 7.0, optimum pH for gelatinase (EC 3.4.23.2) activity.
胰蛋白胨大豆肉汤作为预增菌培养基，在检测人工和天然污染明胶中的沙门氏菌方面优于营养肉汤和乳糖肉汤。当使用明胶酶而非45℃加热来实现明胶 - 肉汤混合物的均质化时，沙门氏菌的检出率进一步提高。通过将明胶 - 肉汤混合物的pH值调节至7.0（明胶酶（EC 3.4.23.2）活性的最佳pH值），检出率也有所提高。
