抗白细胞介素-2受体抗体（达利珠单抗）在肝细胞异种移植实验模型中的有益作用。
Beneficial effect of an antibody against interleukin-2 receptor (daclizumab) in an experimental model of hepatocyte xenotransplantation.
作者信息
Papagoras Dimitrios, Papalois Apostolos, Tsaroucha Alexandra, Lytras Dimitrios, Kyriazanos John, Giannakou Nikoletta, Laftsidis Prodromos, Simopoulos Constantine
机构信息
2nd Department of Surgery, Medical School, Democritus University of Thrace, Alexandroupolis, Greece.
出版信息
World J Gastroenterol. 2007 Mar 7;13(9):1435-7. doi: 10.3748/wjg.v13.i9.1435.
AIM
To investigate the use of Daclizumab (Dmab) as an immunosuppressive agent in an experimental model of hepatocyte xenotransplantation (XenoTx) in rats with fulminant hepatic failure (FHF).
METHODS
Two white male New Zealand rabbits were used as donors and 68 Wistar rats as recipients. FHF was induced by intravenous application of dimethylnitrosamine (DMNA). The isolated hepatocytes of the rabbits were xenotransplanted into the spleen of the rats 24 h after FHF induction. Group A (n = 13): no treatment; Group B (n = 14): FHF and XenoTx; Group C (n = 14): FHF and XenoTx and cyclosporin (CsA); Group D (n = 14): FHF and XenoTx and Dmab; Group E (n = 13): FHF and XenoTx and CsA and Dmab. The rats were followed for 15 d.
RESULTS
Statistical analysis showed better survival among groups D (92.86%) and E (76.92%) compared to group A (all rats died after 72 h), group B (28.57%) or group C (71.43%), although the differences were not statistically significant. Biochemical evaluation of the liver enzymes and histology confirmed satisfactory function and engraftment, respectively.
CONCLUSION
This experimental model has shown the safe, effective and beneficial use of Dmab in a xenotransplantation model of rabbit hepatocytes in rats.
目的
研究达克珠单抗（Dmab）作为免疫抑制剂在暴发性肝衰竭（FHF）大鼠肝细胞异种移植（XenoTx）实验模型中的应用。
方法
以2只白色雄性新西兰兔作为供体，68只Wistar大鼠作为受体。通过静脉注射二甲基亚硝胺（DMNA）诱导FHF。在FHF诱导24小时后，将分离的兔肝细胞异种移植到大鼠脾脏中。A组（n = 13）：未治疗；B组（n = 14）：FHF和XenoTx；C组（n = 14）：FHF和XenoTx及环孢素（CsA）；D组（n = 14）：FHF和XenoTx及Dmab；E组（n = 13）：FHF和XenoTx及CsA和Dmab。对大鼠进行15天的随访。
结果
统计分析显示，与A组（所有大鼠在72小时后死亡）、B组（28.57%）或C组（71.43%）相比，D组（92.86%）和E组（76.92%）的生存率更高，尽管差异无统计学意义。肝酶的生化评估和组织学检查分别证实了功能和植入情况良好。
结论
该实验模型显示了Dmab在大鼠兔肝细胞异种移植模型中的安全、有效和有益应用。
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