Beneficial effect of an antibody against interleukin-2 receptor (daclizumab) in an experimental model of hepatocyte xenotransplantation.

作者信息

Papagoras Dimitrios, Papalois Apostolos, Tsaroucha Alexandra, Lytras Dimitrios, Kyriazanos John, Giannakou Nikoletta, Laftsidis Prodromos, Simopoulos Constantine

机构信息

2nd Department of Surgery, Medical School, Democritus University of Thrace, Alexandroupolis, Greece.

出版信息

World J Gastroenterol. 2007 Mar 7;13(9):1435-7. doi: 10.3748/wjg.v13.i9.1435.

DOI:10.3748/wjg.v13.i9.1435

PMID:17457977

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4146930/

Abstract

AIM

To investigate the use of Daclizumab (Dmab) as an immunosuppressive agent in an experimental model of hepatocyte xenotransplantation (XenoTx) in rats with fulminant hepatic failure (FHF).

METHODS

Two white male New Zealand rabbits were used as donors and 68 Wistar rats as recipients. FHF was induced by intravenous application of dimethylnitrosamine (DMNA). The isolated hepatocytes of the rabbits were xenotransplanted into the spleen of the rats 24 h after FHF induction. Group A (n = 13): no treatment; Group B (n = 14): FHF and XenoTx; Group C (n = 14): FHF and XenoTx and cyclosporin (CsA); Group D (n = 14): FHF and XenoTx and Dmab; Group E (n = 13): FHF and XenoTx and CsA and Dmab. The rats were followed for 15 d.

RESULTS

Statistical analysis showed better survival among groups D (92.86%) and E (76.92%) compared to group A (all rats died after 72 h), group B (28.57%) or group C (71.43%), although the differences were not statistically significant. Biochemical evaluation of the liver enzymes and histology confirmed satisfactory function and engraftment, respectively.